Description
Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.
History of Planar Chromatography.- The Theoretical Basis of Thin-Layer Chromatography (TLC).- The Stationary Phase in Thin Layer Chromatography (TLC).- The Mobile Phase in Adsorption and Partition Chromatography.- Preparing and Applying Samples.- Basis for TLC Development Techniques.- Specific Staining Reactions.- Bioeffective-linked Analysis in Modern HPTLC.- Planar Chromatography Detectors.- Diffuse Reflectance from TLC Layers.- Fluorescence in TLC Layers.- Chemometrics in HPTLC.- Reciprocal Model.- Statistics for Quantitative TLC.- Planning an Analysis and Validation in TLC.
Bernd Spangenberg is a senior professor at Offenburg University of Applied Sciences, Germany. He studied chemistry at Philipps University in Marburg, Germany, where he obtained his PhD in pharmaceutical chemistry. He worked for several years in the pharmaceutical industry in Germany and Switzerland. In 1991, he was appointed professor of environmental analytics at Offenburg University of Applied Sciences, where he has been working since 2021. His research focuses on chromatography and new detection methods in planar chromatography. From 2007 to 2022, he was editor-in-chief of the Journal of Planar Chromatography Modern TLC (a SpringerNature journal).
