Full Description
This book provides an overview of single-cell
isolation, separation, injection,
lysis and dynamics analysis as well as a study of their heterogeneity using
different miniaturized devices. As an important part of single-cell analysis, different
techniques including electroporation,
microinjection, optical trapping, optoporation, rapid electrokinetic patterning
and optoelectronic
tweezers are described in detail. It presents different fluidic
systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry)
and their integration with sensor technology, optical and hydrodynamic stretchers etc.,
and demonstrates the applications of single-cell analysis in systems biology,
proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics,
biomedicine and drug delivery systems. It also discusses the future challenges
for single-cell analysis, including the advantages and limitations.
This book is enjoyable reading material while
at the same time providing essential information to scientists in academia and
professionals in industry working on different aspects of single-cell
analysis.
Dr. Fan-Gang Tseng is a Distinguished Professor of
Engineering and System Science at
the National Tsing Hua University, Taiwan.
Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
Contents
Single
Cell Behavioral Assays for Heterogeneity Studies.- Systems biology in single cells.- Electroporation for single cell
analysis.- Microinjection for single cell
analysis.- Optical tools for single cell
manipulation and analysis.- Optoelectrokinetic manipulation
for cell analysis.- Continuous micro/nano-fluidic
devices for single cell analysis.- Single Cell Mechanical
Properties: Label-Free Biomarkers for Cell Status Evaluation.- Cytometry of single cells for
biology and biomedicine.- Single cell genomics and
epigenomics.- Single cell Metabolomics.- Applications of cell based drug
delivery systems: use of single cell assay.- Applications of Single Cell
Sequencing in Cancer.- Single Cell Characterization of
Microalgal Lipid Contents with Confocal Raman Microscopy.- Single Differentiated Neurons from
Pluripotent Embryonic Stem Cells: Motor Protein Modelling and Neurodegenerative
Disease.
