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Full Description
The development of innovative, efficient ionization techniques allowing a gentle desorption of even complex molecules has provided mass spectrometry with the breakthrough in the direct analysis of polymers. Applicable in various disciplines and yet more powerful than almost any other method, the spectrum of compounds investigated ranges from macromolecular chemistry via biochemistry right up to molecular biology. In this way, suitable mass spectroscopic techniques determine not only the primary structure of proteins but also provide an insight into their spatial structure, even allowing structural and functional analyses. The simplicity of electrospray MS for analyzing non-covalent biomacromolecular complexes opens up brand new perspectives for supramolecular chemistry as well as molecular recognition processes. Furthermore, MS has even found its place in DNA sequencing as a fast but simple alternative to conventional methods.
In his book, the internationally renowned expert, Michael Przybylski, provides a comprehensive overview of this still relatively young methodology, which will soon be as much an integral part of polymer structural analysis as X-ray crystallography and NMR spectroscopy.
Contents
INTRODUCTION: FUNDAMENTALS OF BIOPOLYMER MASS SPECTROMETRY History and instrumental development of biopolymer mass spectrometry Analytical preconditions for mass spectrometry of macromolecules Scope of interdisciplinary application areas IONIZATION METHODS FOR BIOPOLYMER MASS SPECTROMETRY Desorption- Ionisation Methods for Biopolymer Ion Formation - Initial developments Field Desorption 252-Cf Plasma Desorption & Fast Atom Bombardment Matrix-Assisted Laser Desorption- Ionisation (MALDI) Electrospray- Ionisation (ESI) New Developments of Ionisation Techniques INSTRUMENTATION FOR BIOPOLYMER MASS SPECTROMETRY Analysers for Macromolecular Mass Spectrometry Magnetic Sector Analysers Quadrupole Mass Analysers Time-of-Flight Mass Analysers Ion Trap Mass Analysers Hybrid Analysers High Resolution Mass Spectrometers for Biopolymers Fouriertransform- ICR Mass Spectrometry Orbitrap Mass Spectrometry Ion Detection and Spectra Acquisition Preconditions for Macromolecular Ion Detectors Spectra acquisition & data evaluation methods Special High Mass Ion Detectors SEPARATION TECHNIQUES AND HYBRID SYSTEMS FOR BIOPOLYMER MASS SPECTROMETRY Liquid Chromatography- Mass Spectrometry Size Exclusion Chromatography Combination with Gel and Capillary Electrophoresis Bioaffinity- Mass Spectrometry Ion Mobility Mass Spectrometry Online Biosensor- MS METHODS FOR STRUCTURE DETERMINATION OF BIOPOLYMERS Molecular Weight Determination Mechanisms of Biopolymer Ion Formation Mass Resolution and Accuracy of Macromolecular Ions Methods for Primary Structure Determination of Polypeptides Fragmentation of Polypeptide Ions for Tandem-MS Sequence Determination Chemical and Enzymatic Degradation for Sequence Determination Approaches for "Top-Down" Sequence Determination Structural Characterization of Polypeptides by Chemical Modification Combined with Mass Spectrometry Chemical Modification Reactions for Structure Analysis Structural Characterisation using Crosslinking Reactions Analysis of Non-covalent Supramolecular Biopolymer Complexes Preconditions for Analysis of Non-covalent Interactions Specificity Analysis of Biopolymer Complexes Correlation of Solution and Gas Phase Structures of Biopolymer Complexes Characterisation of Higher Order Biopolymer Structures "Charge Structures" of ESI- MS of Biopolymers Ion Mobility- Mass Spectrometry of Polypeptides HD- Exchange Approaches for Probing Biopolymer Interactions Chemical Labelling Approaches for Probing Protein Interaction Structures Characterisation of Biopolymer- Ligand Interactions by Proteolytic Degradation Combined with Mass Spectrometry Fast Photochemical Oxidation of Proteins (FPOP) and Mass Spectrometry BIOCHEMICAL APPLICATIONS OF BIOPOLYMER MASS SPECTROMETRY Introduction Sample Preparation and Handling in Bioanalytical Applications Liquid-Liquid Extraction (LLE) Solid Phase Extraction (SPE) Solid Phase Microextraction and Supercritical Fluid Extraction (SFE) Immunoaffinity Extraction (IAE) & Affinity-Isolation Applications to Primary Structure Analysis of Biopolymers Protein Sequence Determination Sequencing and Mutation Analysis in Polynucleotides Structure Determination of Carbohydrates Mass Spectrometric Proteome Analysis Analysis of Post-Translational Protein Structure Modifications Phosphorylation Glycosylation Fatty Acid Acylation Oxidative Modification of Proteins Ubiquitin Conjugation Selected Applications to Analysis of Biopolymer Interactions Carbohydrate Interactions of Lectins Structure Analysis of Ion Channels by Chemical Modification and Mass Spectrometry BIOMEDICAL APPLICATIONS OF BIOPOLYMER MASS SPECTROMETRY Analysis of Recognition Structures in Molecular Immunology Identification of Polypeptide Antigen Epitopes Identification of Antibody Paratopes Characterization of Oligomerization- Aggregation Pathways in Target Proteins of Neurodegenerative Diseases Applications to Clinical Diagnostics of Lysosomal Storage Diseases Enzymes Clinical Applications of Affinity- Mass Spectrometry to Autoimmune Diseases PERSPECTIVES OF BIOPOLYMER MASS SPECTROMETRY
