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Full Description
For the first time, this promising technology is presented in a readily accessible manner. The two editors, a method developer and a method user, have tailored the book to the needs of occasional or first-time users, providing ample practical advice at every stage, and including a detailed walk-through example of a real-life protein study. The result is a hands-on guide starting with basic considerations of the advantages and disadvantages of this relatively new technology, before going on to discuss in practical terms the course of the diffraction study, from sample preparation via the actual measurement to the collection and analysis of the diffraction data. For biochemists, biophysicists, crystallographers, medicinal chemists, and radiation physicists.
Contents
INTRODUCTION Historical Introduction Scope of Neutron Protein Crystallography Complementarity to other Techniques Advantages and Disadvantages Future Trends Planning a Neutron Study SAMPLE PREPARATION Planning Sample Preparation Cloning, Expression and Perdeuteration Crystallization and Solvent Exchange Room Temperature and Cryo Sample Techniques DATA COLLECTION Planning Data Collection Monochromatic Techniques Quasi-Laue Techniques Time-of-Flight Techniques DATA REDUCTION Monochromatic Data Quasi-Laue Techniques Time-of-Flight Techniques STRUCTURE DETERMINATION AND ANALYSIS Planning Structure Refinement PHENIX nCNS Shelx HDX Analyses Water Building Determining Protonation States Hydrogen Bond Analyses Depositing Structures WALK-THROUGH EXAMPLES PCS: DFPase ADI D19: XI BIX BioDiff APPENDICES
