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Full Description
Unique in its scope and approach, this two-volume handbook systematically surveys the wide range of current methods for chemical compound testing in drug research. Covering everything from low-throughput to medium- and high-throughput assays, and spanning both biological, chemical and physical assay principles, this ready reference explains how to improve the use of existing assay equipment while also demonstrating the alternative technology available for any particular assay need. Volume One is organized by assay principle, allowing current users to expand and improve their range of assay options while showing potential users whether it is worthwhile investing in the required equipment. The second volume is organized by target protein class, thus mirroring the view of a drug developer charged with establishing a new assay platform for a designated target. The editor and authors are working in the most advanced assay labs within the pharmaceutical and biotech industry, including Novartis, Roche, Glaxo, Pfizer, Bayer, Lilly, Boehringer Ingelheim, Abbott, and Wyeth, providing first-hand knowledge of the latest technology currently on the market.
Should be on the desk of every drug discovery scientist in academia and industry.
Contents
VOLUME I: READOUT TECHNOLOGIES HPLC Technologies Microfluidics Single-Molecule Spectroscopy High-Content Screening Surface-Plasmon Resonance Spectroscopy Microcalorimetry FLIPR Technology Affinity-Selection of Binders Mass-Spectrometry Readouts Protein Complementation Techniques Radiometric Assay Technologies Fluorescence Intensity Fluorescence Polarisation Fluorescence Lifetime Time-resolved FRET ALPHA Screen Reverse Protein Arrays Cell Impedance Measurements ELISA Electrophysiology Rubidium Efflux Technologies Yeast Two-Hybrid System Reporter Gene Assays VOLUME II: TARGET FAMILIES Ion Channels and Transporters Drug Transporters Voltage-gated Ion Channels G Protein-coupled Receptors Kinases Proteases Methyltransferases Helicases and Gyrases Acetylases and Deacetylases GTPases Phosphodiesterases Nuclear Hormone Receptors Reverse Transcriptases and Polymerases Transcription Factors Enyzme Activators Multienzyme Complexes Protein-Protein Interactions
