基本説明
Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.
Full Description
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.
Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
Contents
Preface
Foreword
Abbreviations
Glossary of terms
INTRODUCTION
History
Critical points
Hypothesis driven approach vs. High Throughput
Complementarities of workflows
Concept of experimental planning
Success stories, highlights of useful references
PART I: PROTEOMICS TECHNOLOGY
Electrophoresis
Liquid Chromatography
Mass spectrometry
Bioinformatics tools / data mining
Methods under development
PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS
Strategy where to start
Gel-based workflow
LC-based workflow
Latest trends
Validation with Western blotting
Statistic evaluation
PART III: TROUBLE SHOOTING
Gel-based workflow
LC based workflow
References
Index
