基本説明
Written by one of the most significant contributors to the progress of protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation.
Full Description
Written by one of the most significant contributors to the progress of protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation. It covers the theory, practice and latest achievements in x-ray crystallography, such that any researcher in structural biology will benefit from this extremely clearly written book.
Part A covers the theoretical basis and such experimental techniques as principles of x-ray diffraction, solutions for the phase problem and time-resolved x-ray crystallography. Part B includes case studies for different kinds of x-ray crystal structure determination, such as the MIRAS and MAD techniques, molecular replacement, and the difference Fourier technique.
Contents
PART A THEORETICAL BASIS AND EXPERIMENTAL TECHNIQUES
Introduction
Techniques
Principles of X-ray Diffraction by a Crystal
Methods for Solving the Phase Problem
Model Building and Refinement
Time-resolved X-ray Crystallography
PART B CASE STUDIES FOR DIFFERENT KINDS OF X-RAY CRYSTAL STRUCTURE DETERMINATION OF BIOMACROMOLECULES
Space Group Determination and Data Reduction
Phase Determination by MIRAS Technique
Phase Determination by MAD Technique
Phase Determination by Molecular Replacement
Difference Fourier Technique
Map Improvement by NCS Averaging and other Methods
Automated Model Building
Structure Validation and PDB-Submission
