Full Description
This book describes in 13 chapters mechanisms of P450 used to monooxygenate substrates via the NAD(P)H/O2 pathway using its peroxidase and peroxygenase functions. P450 also utilizes peroxides, peracids, periodate and iodosobenzene to oxygenate substrates via the shunt pathway. Also described are mechanisms used in the oxidation of pharmaceuticals by CYP3A4; acyl- carbon cleavage by CYP17A1, CYP19A1 and CYP51A1; metabolism of tetrabromodiphenyl ethers and bile acids by CYP2B6 and CYP3A4; metabolism of ω-6 and ω-3 polyunsaturated fatty acids; H2O2-mediated peroxygenation of substrates using substrate misrecognition; P450 oxidative reactions using electrochemical methods; electron transfer to P450 by redox proteins; hydroxylation of 1,8-cineole by P450cin; and peroxygenation by unspecific peroxygenases using H2O2. The topics covered are relevant to P450 researchers, professors and students from a variety of disciplines ranging from pharmacology, toxicology and microbiology to chemistry.
Contents
Foreword.- 1. Monooxygenase, Peroxidase and Peroxygenase Properties and Reaction Mechanisms of Cytochrome P450 Enzymes .- 2. Oxidizing Intermediates in P450 Catalysis: A Case for Multiple Oxidants.- 3. Current Approaches for Investigating and Predicting Cytochrome P450 3A4-Ligand Interactions.- 4. Acyl-Carbon Bond Cleaving Cytochrome P450 Enzymes: CYP17A1, CYP19A1 and CYP51A1.- 5. Regioselective Versatility of Monooxygenase Reactions Catalyzed by CYP2B6 and CYP3A4: Examples With Single Substrates.- 6. Cytochrome P450 Enzymes in the Bioactivation of Polyunsaturated Fatty Acids and their Role in Cardiovascular Disease.- 7. Monooxygenation of Small Hydrocarbons Catalyzed by Bacterial Cytochrome P450s.- 8. Use of Chemical Auxiliaries to Control P450 Enzymes for Predictable Oxidations at Unactivated C-H Bonds of Substrates,- 9. Cytochrome P450 Enzymes and Electrochemistry: Crosstalk with Electrodes as Redox Partners and Electron Sources.- 10. Mechanistic Basis of Electron Transfer to Cytochromes P450 by Natural Redox Partners and Artificial Donor Constructs .- 11. Biological Diversity of Cytochrome P450 Redox Partner Proteins .- 12. Cytochrome P450cin (CYP176A1).- 13. Fungal Unspecific Peroxygenases: Heme-Thiolate Proteins that Combine Peroxidase and Cytochrome P450 Properties.
