Full Description
This book pinpoints one of the fastest growing, complex subjects in chemistry and medical science: the dangers of oxidative stress to human beings. It provides a solid background on the chemistry behind the generation of reactive species as well as how reactive species are involved in essential physiological processes and in almost every human disease. It also covers the most recent developments in the study of oxidative and reductive stress (redox stress), including the role of radical and reactive species, novel antioxidant therapies, and methods for assessing free radicals and redox stress. The chapters present concise, yet thorough, summaries of the state-of-the-art methods and techniques that any investigator working in the oxidative/reductive stress field needs to access. The current methodologies including the development of sensors and biosensors for the detection of ROS/RNS/RHS and of biomarkers of redox stress are thoroughly discussed. This book is a useful resource for all researchers and students interested in oxidative stress, molecular biology, and chemistry. Physicians and healthcare professionals interested in understanding the molecular mechanisms underlying the redox stress-related diseases also stand to benefit from this book.
Contents
Chapter 1 - Chapter 1: Introduction: Physicochemical properties and physiological roles of ROS/oxidative eustress and distress.- Section 1 - Classifications of oxidative stress biomarkers.- Chapter 2 - Reactive species.- Chapter 3 - Biomarkers of oxidative damage.- Chapter 4 - Pro-oxidants.- Chapter 5 - Antioxidants.- Chapter 6 - Other biomarkers.- Section 2: Conventional and advanced measurement techniques of oxidative stress.- Chapter 7 - Introduction.- Chapter 8 - Spectrophotometric methods.- Chapter 9 - Chemiluminescence methods.- Chapter 10 - Fluorimetric methods.- Chapter 11 - Fluorescence-based imaging techniques.- Chapter 12 - Electron paramagnetic resonance/Electron spin resonance.- Chapter 13 - Chapter 13: Electrochemical Methods and Sensors.- Chapter 14 - Enzymatic methods.- Chapter 15 - Chromatographic methods.- Chapter 16 - Immunoassays/ELISA.- Chapter 17 - Immunohistochemistry.- Chapter 18 - MiOXSYS.- Chapter 19 - TUNEL Assay and SCSA.- Chapter 20 - Proteomics.- Chapter 21 - Other techniques for oxidative stress assessment.- Chapter 22 - Oxidative stress monitoring: needs and opportunities.- Chapter 23 - Future directions, pitfalls and solutions in oxidative stress assessment.
