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Full Description
This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications.
Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process.
This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.
Contents
Chapter 1. General Principles.- Chapter 2. Signals - Scattering.- Chapter 3. Signals - Fluorescence, Phosphorescence, Impedance, Extinction.- Chapter 4. Fluidics.- Chapter 5. Light Sources.- Chapter 6. Optical Benches.- Chapter 7. Detectors and Electronics.- Chapter 8. Signal Analysis.- Chapter 9. The Cytometric File.- Chapter 10. Data Transformation.- Chapter 11. Data Representation.- Chapter 12. Data Analysis.- Chapter 13. Standards, Setup, Calibration, and Control Techniques.- Chapter 14. Fluorochromes - Overview.- Chapter 15. Fluorochromes Suitable for Antibody Conjugation.- Chapter 16. Fluorochromes That Bind Nucleic Acids.- Chapter 17. Fluorochromes for the Study of the Cell Features.- Chapter 18. Fluorescent Proteins.- Chapter 19. Spillover and Compensation.- Chapter 20. Artifacts.- Chapter 21. Cell Sorting.- Chapter 22. Non-Conventional Flow Cytometry.- Chapter 23. Statistics: A Cytometric Point of View.
