Full Description
This atlas provides a detailed overview of the novel technique of ex vivo confocal microscopy for rapid imaging of excised tissues in dermatological practice. It features an extensive collection of ex vivo images acquired from normal skin structures and from a variety of neoplastic lesions (benign and malignant) and inflammatory lesions. Each chapter contains several image types of a particular disorder, including gray-scale, digital purple-pink images (DHE) and hematoxylin and eosin (H&E) correlations to assist the acquisition of diagnostic skills. Guidance on how to use techniques for tissue preparation, staining, handling and image acquisition are also provided enabling the reader to develop confidence in integrating this technique into their day-to-day practices. Furthermore, this atlas also provides an update on the ongoing latest advances in the field.
Cutaneous Atlas of Ex Vivo Confocal Microscopy covers how to apply these techniques into dermatological practice, especially in Mohs surgery for the evaluation of keratinocytic neoplasm and in dermatopathology for rapid evaluation of varied skin lesions. It is therefore a valuable resource for trainee, residents, practicing dermatologists and dermatopathologists who are seeking a resource to assist in developing their knowledge and skills of utilizing these methodologies.
Contents
Section I. Basics of Ex Vivo Confocal Microscopy.- Roles and Applications of Ex Vivo Confocal Microscopy.- Principles of Ex Vivo Confocal Microscopy.- Hands-On Guide for Ex Vivo Confocal Imaging.- Section II. Normal Skin.- Normal Skin on Ex Vivo Confocal Microscopy: Patterns and Histopathologic Correlation.- Section III. Benign Skin Tumours.- Features of Benign Epidermal Nonmelanocytic Lesions on Ex Vivo Confocal Microscopy and Histopathologic Correlation.- Features of Benign Nonmelanocytic Dermal and Subcutaneous Lesions on Ex Vivo Confocal Microscopy and Histopathologic Correlation.- Section IV. Malignant Keratinocyte Neoplasms.- Morphological Features of Basal Cell Carcinoma on Ex Vivo Confocal Imaging and Histopathologic Correlation.- Squamous Cell Carcinoma Features on Ex Vivo Confocal Imaging and Histopathologic Correlation.- Section V. Melanocytic Lesions.- Normal Nevi: Junctional, Compound, and Dermal.- Dysplastic Nevi.- Melanoma.- Section VI. Inflammatory Lesions.- Featuresof Lichen Planus and Psoriasis on Ex Vivo Confocal Imaging and Histopathologic Correlation.- Eczema: Features on FCM, Digital H&E, and Corresponding Conventional H&E.- Fluorescence Confocal Microscope for Identification of Discoid Lupus Erythematosus Histologic Features.- Immunofluorescence with Confocal Microscopy: Pemphigoid, Pemphigus, Cutaneous Vasculitis, Lichen Planus, and Cutaneous Lupus Erythematosus.- Section VII. Future of Ex Vivo Confocal Microscopy.- Advances in Technology, Staining Protocol, and Flattening Devices in Ex Vivo Confocal Microscopy.- Epidermal Reconstruction During Ex Vivo Confocal Microscopy for Detection of Superficial Basal Cell Carcinoma with 3D-mosaicking and Intensity Projection.
