Full Description
Various "omics" methods have recently revolutionized molecular diagnostics. Next-generation sequencing (NGS) makes it possible to sequence a human genome in just one day. Whole genome sequencing (WGS) greatly improves the ability to investigate the outbreaks of numerous pathogens. Metagenomics helps to analyze the microbiome, which aids greatly in identifying the pathogenesis of infectious diseases. Proteomic-based methods, namely matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF-MS), have a promising role in identifying myctobacteria and fungi, and predicting antimicrobial resistance.
While there are numerous scientific publications on "omics" applications for microbiology, there are relatively few books that review this topic from a clinical diagnostics perspective. This book looks at this field from a holistic viewpoint, instead of limiting by type of "omics" technology, in order to cover the body of knowledge needed for practitioners andacademics interested in clinical and public health microbiology. Additionally, it addresses the management, economical, regulatory and operational aspects of integrating these technologies into routine diagnostics.
Contents
Ch 1: Overview of Microbial NGS.- Ch 2: WGS for Bacterial Identification and Susceptibility Testing.- Ch 3: WGS for Bacterial Virulence Assessment.- Ch 4: Epidemiological Typing by WGS .- Ch 5: Bioinformatics Approaches for WGS Analysis.- Ch 6: WGS for Clinical Virology.- Ch 7: WGS for Clinical Mycology.- Ch 8: WGS for Clinical Parasitology .- Ch 9: Long Read Sequencing.- Ch 10: Aspects of WGS Integration.- Ch 11: Microbiome Analysis.- Ch 12: Metagenomics of Environmental Samples.- Ch 13: Clinical Metagenomics .- Ch 14: Metagenomics for Pathogen Discovery.- Ch 15: Advanced Applications for MALDI-TOF: Typing.- Ch 16: Advanced Applications for MALDI-TOF: Infectious Diseases and Antibiotic Susceptibility Testing (AST).- Ch 17: Omics for Food and Water Microbiology.- Ch 18: Omics for Forensic and Post-Mortem Microbiology.- Ch 19: Omics for Antimicrobial Resistance Surveillance.- Ch 20: Total Laboratory Automation.
