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Full Description
This book provides theoretical, practical and troubleshooting guidelines on biochemical methods in high-throughput screening as part of the pre-clinical Drug Discovery process. There is particular focus on the design, development and role of screening assays in Drug Discovery, including new developments and future trends, the main assay types utilized in screening campaigns, and the methods utilized in enabling the progression of "Hits" to "Leads" and "Candidates".The book will cover colorimetric, fluorescence resonance energy transfer, luminescence, scintillation proximity and enzyme-linked immunosorbent assays in detail, and contains comprehensive appendices providing detailed information on commercially available assays currently available.This text will provide an invaluable guide for biochemists and medicinal chemists working in the pharmaceutical industry and academia.
Contents
Volume 1Fluorescence intensity; Fluorescence polarisation; FRET, TR-FRET/HTRF; AlphaScreen; Fluorescence lifetime; Scintillation Proximity Assay; Chip based assays; Label free assays; Calorimetry; ELISA; Volume 2: Cell Based Methods in High Throughput Screening: Fluorescence intensity; FRET, TR-FRET/HTRF; AlphaScreen; Reporter gene assays; Luminescence & Bioluminescence; Scintillation Proximity Assay; Calcium mobilization (FLIPR); cAMP assays; Electrophysiology; Cellular imaging & fluorescent microscopy; Label free assays; Volume 3: Pre-Clinical Drug Discovery, High Throughput Screening and Compound Progression Strategies: The Drug Discovery process; Target classes and validation; Assays in Drug Discovery: biochemical vs cellular; Enzyme kinetics; Receptor kinetics in drug screening programmes; Biological reagents for High Throughput Screening; Automation vs manual operation of screening tasks; Analysis of High Throughput Screening data; Compound Mechanism of Action studies; Counter, Selectivity, Viability and Liability assays; Screening for undesirable compounds; Toxicology profiling; Compound characterization: physico-chemical properties; Index
