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Full Description
Detection of Pathogens in Water Using Micro and Nano-Technology aims to promote the uptake of innovative micro and nano-technological approaches towards the development of an integrated, cost-effective nano-biological sensor useful for security and environmental assays.
The book describes the concerted efforts of a large European research project and the achievements of additional leading research groups. The reported knowledge and expertise should support in the innovation and integration of often separated unitary processes. Sampling, cell lysis and DNA/RNA extraction, DNA hybridisation detection micro- and nanosensors, microfluidics, together also with computational modelling and risk assessment can be integrated in the framework of the current and evolving European regulations and needs. The development and uptake of molecular methods is revolutionizing the field of waterborne pathogens detection, commonly performed with time-consuming cultural methods. The molecular detection methods are enabling the development of integrated instruments based on biosensor that will ultimately automate the full pathway of the microbiological analysis of water.
Editors: Giampaolo Zuccheri, University of Bologna, Italy and Nikolaos Asproulis, Cranfield University, UK
Contents
Contents: Overview of European regulation and standards on microbiological water analysis; Risk analysis of bio-terroristic attacks on drinking water systems; Sample collection procedures for Online Contaminant Monitoring System; A device to extract highly diluted specimens out of large volumes of water for analysis in lab-on-a-chip detection systems; Sustainable DNA/RNA release methods for in-line waterborne pathogen screening devices; The microsystem based core of the DINAMICS water testing system: Design considerations and realization of the chip units; Electrochemical biosensor strategies for pathogen detection in water security; Biochemical and Nanotechnological Strategies for Signal Enhancement in the Detection of Nucleic Acids with biosensors; Computational modelling of aqueous environments in micro and nanochannels; Computational recipes of transport phenomena in micro and nanofluidics; Multi-detection of waterborne pathogens in raw and treated water samples by using ultrafiltration concentration and DNA array technology; Detection and enumeration of waterborne mycobacteria; New molecular technologies for the rapid detection of Legionella in water; Detection of virus in the water environment; Design of PCR primers for the detection of waterborne bacteria; Fluid structure and boundary slippage in nanoscale liquid films; Understanding slip at the nanoscale in fluid flows using atomistic simulations
