CRISPR/Cas-Mediated Genome Editing in Plants

Pandita, Deepu (EDT)/ Pandita, Anu (EDT)

Apple Academic Press Inc.（2023/06発売）

• 製本 Hardcover:ハードカバー版／ページ数 458 p.
• 言語 ENG
• 商品コード 9781774912126
• DDC分類 631.5233

Full Description

With over 820 million people facing hunger in today's world, the need of the hour is the design of plant varieties with high yield, improved traits, and resistance properties in order to mitigate the detrimental impacts of biotic and abiotic stress conditions on food crops. This volume highlights the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated nuclease proteins as a solution for sustainable agriculture. CRISPR/Cas-mediated genome editing (GE) provides a robust, simple, precise, efficient, economical, and revolutionary toolbox that leads to improved plant traits with enhanced yield, quality, and resistance against various stresses, including climate-related stress, insect pests and diseases, pollution-related stresses, etc.

Key features of the book:

Explains the differences between conventional breeding, genetically modified crops, and genome editing approaches using CRISPR/Cas
Introduces the tools of genome editing, such as ZFNS (zinc finger nucleases), mega nucleases, and TLENS (transcription activator-like effector nucleases)
Covers the history, origin, discovery, structure, and classification of CRISPR/Cas
Examines the databases and computational approaches of CRISPR/Cas
Highlights genome editing of important crop plants with CRISPR/Cas systems, including wheat, maize. tomato, Brassica crops, rice, fruits
Explores potential applications of CRISPR/Cas systems for climate smart crops

Contents

PART I: INTRODUCTION TO GENOME EDITING TOOLS 1. Conventional Breeding, Genetically Modified Crops, and Genome Editing 2. An Overview of Tools for Genome Editing: ZFNs, Mega Nucleases, and Talens 3. Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated (CRISPR/Cas) Systems: Discovery, Structure, Classification, and General Mechanism 4. Computational Tools and Approaches for CRISPR-Cas Technology PART II: GENOME EDITING OF CROP PLANTS WITH CRISPR/CAS SYSTEMS: PAST, PRESENT, AND FUTURE 5. Genome Editing Using CRISPR/Cas9 System for Crop Improvement, Nutrient Enrichment, and Abiotic Stress Tolerance in Rice 6. Genome Editing of Wheat with CRISPR/Cas Systems: Past, Present, and Future 7. Genome Editing of Maize with CRISPR/Cas System: Past, Present, and Future 8. Genome Editing of Tomato Crop with CRISPR/Cas Systems: Past, Present, and Future 9. Genome Editing Techniques and Their Applications in Brassica Crops Improvement PART III: POTENTIAL APPLICATIONS OF CRISPR/CAS SYSTEMS FOR CLIMATE SMART CROPS 10. CRISPR/Cas-Mediated Genome Editing for Abiotic Stress Tolerance 11. CRISPR/Cas-Mediated Genome Editing for Biotic Stress Tolerance 12. CRISPR/Cas-Mediated Genome Editing for Improvement of Plant Traits 13. Investigation of Plant-Microbe Interactions by CRISPR/Cas for Sustainable Agriculture 14. Unravelling the Application of CRISPR/Cas System in Natural Product Research 15. Unravelling the Promise of CRISPR/Cas System in Modulating Nutrients and Antinutrients in Fruits 16. CRISPR-Cas-Based Strategies for Resistance against Plant Viruses 17. Regulatory Status of CRISPR/Cas Genome-Edited Crops