Full Description
The evaluation of potential mutagenic activity is a critical step in the assessment of the safety of both new and pre-existing chemical types. In Genetic Toxicology: Principles and Methods, expert contributors help to satisfy the demand for education in this tremendously important area of study. The volume covers three basic areas: the scientific basis of the discipline, the methodologies of the main test assays, and the application of the methods, all aimed primarily at scientists in the safety departments of the industries working with both natural and synthetic chemicals. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Intuitive and cutting-edge, Genetic Toxicology: Principles and Methods provides crucial support to both laboratory workers in providing quality information on the appropriate application of techniques and to study directors in their assay selection and protocol design in this vital field.
Contents
The Application of Structure Activity Relationships to the Prediction of the Mutagenic Activity of Chemicals.- Bacterial Mutagenicity Assays: Test Methods.- The Mouse Lymphoma Assay.- Mammalian Cell HPRT Gene Mutation Assay: Test Methods.- The In Vitro Mammalian Chromosome Aberration Test.- The Interpretation and Analysis of Cytogenetic Data.- The In Vitro Micronucleus Assay.- The In Vitro and In Vivo Comet Assays.- Assessment of DNA Interstrand Crosslinks (ICLs) Using the Modified Alkaline Comet Assay.- 32P-Postlabelling for the Sensitive Detection of DNA Adducts.- Methods for the Detection of DNA Adducts.- The GADD45a-GFP GreenScreen HC Assay.- Real Time Reverse-Transcription Polymerase Chain Reaction (RT-PCR): Technical Considerations for Gene Expression Analysis.- Cytogenetic In Vivo Assays in Somatic Cells.- Cytogenetic Methods in Human Biomonitoring: Principles and Uses.- The Measurement of Induced Genetic Change in Mammalian Germ Cells.- Transgenic Animal Mutation Models: A Review of the Models and How They Function.- Analysis of Genotoxicity Data in a Regulatory Context.
