Chloroplast Research in Arabidopsis : Methods and Protocols (Methods in Molecular Biology) 〈Vol. 775〉

個数:

Chloroplast Research in Arabidopsis : Methods and Protocols (Methods in Molecular Biology) 〈Vol. 775〉

  • 提携先の海外書籍取次会社に在庫がございます。通常3週間で発送いたします。
    重要ご説明事項
    1. 納期遅延や、ご入手不能となる場合が若干ございます。
    2. 複数冊ご注文の場合は、ご注文数量が揃ってからまとめて発送いたします。
    3. 美品のご指定は承りかねます。

    ●3Dセキュア導入とクレジットカードによるお支払いについて
  • 【入荷遅延について】
    世界情勢の影響により、海外からお取り寄せとなる洋書・洋古書の入荷が、表示している標準的な納期よりも遅延する場合がございます。
    おそれいりますが、あらかじめご了承くださいますようお願い申し上げます。
  • ◆画像の表紙や帯等は実物とは異なる場合があります。
  • ◆ウェブストアでの洋書販売価格は、弊社店舗等での販売価格とは異なります。
    また、洋書販売価格は、ご注文確定時点での日本円価格となります。
    ご注文確定後に、同じ洋書の販売価格が変動しても、それは反映されません。
  • 製本 Hardcover:ハードカバー版/ページ数 430 p./サイズ 64 illus.
  • 言語 ENG
  • 商品コード 9781617792366
  • DDC分類 576

基本説明

Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis.

Full Description

Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable.  The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid.  In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Contents

One- and Two-Dimensional Blue Native (BN) PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes.- Analysis of Thylakoid Protein Complexes by Two Dimensional Electrophoretic Systems.- Preparation of Multiprotein Complexes from Arabidopsis Chloroplasts Using Tandem Affinity Purification.- Studying Interactions between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation (BiFC) and Förster Resonance Energy Transfer (FRET).- Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods.- Proteome Databases and Other Online Resources for Chloroplast Research in Arabidopsis.- Use of Transcriptomics to Analyse Chloroplast Processes in Arabidopsis.- Use of Non-Aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis.- Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis.- Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies.- Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis.- Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies.- Preparation and Proteomic Analysis of Chloroplast Ribosomes.- The Workflow for Quantitative Proteome Analysis of Chloroplast Development and Differentiation, Chloroplast Mutants, and Protein Interactions by Spectral Counting.- Use of Phosphoproteomics to Study Post-Translational Protein Modifications in Arabidopsis Chloroplasts.- Use of a Pulse-Amplitude Modulated (PAM) Chlorophyll Fluorometer to Study the Efficiency of Photosynthesis inArabidopsis Plants.- Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves.- Measurement of the ΔpH and Electric Field Developed Across Arabidopsis Thylakoids in the Light.- Measurement of Chloroplast ATP Synthesis Activity in Arabidopsis.- Methods for Analysis of Photosynthetic Pigments and Steady-State Levels of Intermediates of Tetrapyrrole Biosynthesis.- Analysis of Starch Metabolism in Chloroplasts.- The Analysis of Lipid Content and Quality in Arabidopsis Plastids.

最近チェックした商品