Full Description
Arthritis Research: Methods and Protocols is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover such exciting and cutting edge topics as imaging and immunohistochemistry, analysis of cartilage and bone catabolism, immunobiology, and cell trafficking.
In Volume 1, authors discuss synovial joint morphology, histopathology, and immunohistochemistry, cartilage matrix and bone biology, and cell trafficking, migration and invasion. Volume 2 is broken up into sections that cover immunobiology and T cells, animal models of arthritis, and applications of new technologies, such as Differential Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR), to define novel therapeutic targets. Both volumes combine to produce a concise set of protocols condensing decades of experience and expertise.
Arthritis Research: Methods and Protocols will be a valuable tool for basic research investigators, clinician scientists, pathologists, immunologists, and biochemists looking to stay current in their fields.
Contents
Synovial Joint Morphology, Histopathology, and Immunohistochemistry.- Imaging Inflamed Synovial Joints.- Arthroscopy as a Research Tool.- Immunohistochemistry of the Inflamed Synovium.- In Situ Hybridization of Synovial Tissue.- Subtractive Hybridization.- Laser Capture as a Tool for Analysis of Gene Expression in Inflamed Synovium.- Preparation of Mononuclear Cells from Synovial Tissue.- Quantitative Image Analysis of Synovial Tissue.- Cartilage Matrix and Bone Biology.- Cartilage Histomorphometry.- Image Analysis of Aggrecan Degradation in Articular Cartilage With Formalin-Fixed Samples.- In situ Detection of Cell Death in Articular Cartilage.- Measurement of Glycosaminoglycan Release from Cartilage Explants.- Assessment of Collagenase Activity in Cartilage.- Assessment of Gelatinase Expression and Activity in Articular Cartilage.- Analyses of MT1-MMP Activity in Cells.- Analysis of TIMP Expression and Activity.- Bone Histomorphometry in Arthritis Models.- Generation of Osteoclasts In Vitro, and Assay of Osteoclast Activity.- Cell Trafficking, Migration, and Invasion.- Isolation and Analysis of Large and Small Vessel Endothelial Cells.- Analysis of Flow-Based Adhesion In Vitro.- Analysis of Leukocyte Recruitment in Synovial Microcirculation by Intravital Microscopy.- Angiogenesis in Arthritis.- Analysis of Inflammatory Leukocyte and Endothelial Chemotactic Activity.- Acquisition, Culture, and Phenotyping of Synovial Fibroblasts.- Genotyping of Synovial Fibroblasts.- Gene Transfer to Synovial Fibroblast.- In Vitro Matrigel Fibroblast Invasion Assay.- Culture and Analysis of Circulating Fibrocytes.
