Full Description
The detrimental impacts of pain on the quality of our daily life have drawn increasing attention from researchers, health care providers, policymakers, and social workers. The reality of effective painkillers specifically designed for different types of pain states has been obscured by missing knowledge of the mechanisms of different types of pain. Thus, studying the complexity of pain transduction, which includes various insults to the peripheral nervous systems, sensitized spinal circuits, and altered signals ascending to or descending from the brain, has emerged as a high priority task on the agenda of pharmaceutical companies and other private as well as public agencies. To accomplish this mission, one requires a combination of well-integrated systems, such as a- mal models resembling the pathological conditions of pain transduction, and an understanding of the interactions among pain transducers and mediators at the molecular level. Thanks to rapid advancements in the development of novel cellular and molecular biology techniques, as well as in our understanding of physiology, and of the behavioral pharmacology of pain transduction, the time is now ripe for dissecting the molecular mechanisms of pain transduction using multidisciplinary approaches. Indeed, my acceptance of the invitation from the series editor, Dr. John Walker, to assemble a book of methods and protocols for pain research was inspired by these emerging needs. The purpose of Pain Research: Methods and Protocols is to provide st- by-step methods and protocols of multidisciplinary approaches related to the study of pain transduction.
Contents
Mechanistic Dissection of Pain: From DNA to Animal Models
Z. David Luo
Assessment of Acute Thermal Nociception in Laboratory Animals
Jeffrey W. Allen and Tony L. Yaksh
Tissue Injury Models of Persistent Nociception in Rats
Jeffrey W. Allen and Tony L. Yaksh
Segmental Spinal Nerve Ligation Model of Neuropathic Pain
Jin Mo Chung, Hee Kee Kim, and Kyungsoon Chung
Partial Sciatic Nerve Transection
Thies Lindenlaub and Claudia Sommer
Modeling Diabetic Sensory Neuropathy in Rats
Nigel A. Calcutt
Sciatic Inflammatory Neuropathy in the Rat: Surgical Procedures, Induction of Inflammation, and Behavioral Testing
Erin D. Milligan, Steven F. Maier, and Linda R. Watkins
A Rat Pain Model of Vincristine-Induced Neuropathy
Emiliano S. Higuera and Z. David Luo
Loop Dialysis Catheter: A Technology for Chronic Spinal Dialysis in a Freely Moving Rat
Martin Marsala
Intrathecal Catheterization and Drug Delivery in the Rat
Shelle A. Malkmus and Tony L. Yaksh
Trigeminal Neuronal Recording in Animal Models of Orofacial Pain
Koichi Iwata, Yuji Masuda, and Ke Ren
In Vivo Electrophysiology of Dorsal-Horn Neurons
Louise C. Stanfa and Anthony H. Dickenson
Single-Fiber Recording: In Vivo and In Vitro Preparations
Maria Schäfers and David Cain
Anatomical Identification of Neurons Responsive to Nociceptive Stimuli
Luc Jasmin and Peter T. Ohara
Isolation and Culture of Sensory Neurons From the Dorsal-Root Ganglia of Embryonic or Adult Rats
Thomas H. Burkey, Cynthia M. Hingtgen, and Michael R. Vasko
Primary Cultures of Neonatal Rat Spinal Cord
Virginia S. Seybold and Lia G. Abrahams
Single-Cell Laser Capture Microdissection and RNA Amplification
Fredrik Kamme, Jessica Zhu, Lin Luo, Jingxue Yu, Da-Thao Tran, Bernhard Meurers, Anton Bittner,Karin Westlund, Susan Carlton, and Jackson Wan
Semi-Quantitative Real-Time PCR for Pain Research
Hong Qing Guo and Sandra R. Chaplan
Functional Genomic Analysis in Pain Research Using Hybridization Arrays
Stephen J. Walker, Travis J. Worst, Willard M. Freeman, and Kent E. Vrana
Generation of Transgenic Mice
Guoping Feng, Jing Lu, and Jimmy Gross
Knockout Mouse Models in Pain Research
Andrée Dierich and Brigitte L. Kieffer
Index
