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Full Description
This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro vided for individual analyses. The intention is to provide general guide lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.
Contents
General Guidelines to the Operation of Capillary Electrophoresis Methods and Instrumentation.- Fundamentals of Capillary Electrophoresis Theory.- Standard Commercial Instrument Description.- Typical Operating Procedures.- Method Development/Optimization.- Quantitation Procedures.- Optimization of Precision in Quantitative Analysis.- Optimization of Sensitivity.- Method Validation.- Fraction Collection.- Troubleshooting.- Quick Guide to Running a Successful Separation.- Applications of Capillary Electrophoresis and Specific Technologies.- Micellar Electrokinetic Chromatography.- Capillary Gel Electrophoresis.- Chiral Separations by Capillary Electrophoresis.- Capillary Electrochromatography.- Application and Limits of Sample Stacking in Capillary Electrophoresis.- Analysis of Bases, Nucleosides, and (Oligo)nucleotides by Capillary Electrophoresis.- Application of Capillary Electrophoresis to Pharmaceutical Analysis.- Separation of Peptides and Protein Digests by Capillary Electrophoresis.- Additional Application Areas of Capillary Electrophoresis.
