Full Description
DNA methylation is a type of chemical modification of DNA that can be inherited without changing the DNA sequence. As such, it is part of the epigenetic code and is the most characterised epigenetic mechanism. DNA methylation involves the addition of a methyl group to DNA -- for example, to the number 5 carbon of the cytosine pyrimidine ring. DNA methylation is probably universal in eukaryotes. In humans, approximately 1% of DNA bases undergo DNA methylation. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells.In plants, cytosines are methylated both symmetrically (CpG or CpNpG) and asymmetrically (CpNpNp), where N can be any nucleotide. The methylation status of specific cytosines can be determined using methods based on bisulfite sequencing. This book presents leading-edge research advances in the field.
Contents
Preface; Research and Review Articles; Peculiarities of DNA Methylation in Plants; New and Improved Methods for Analysis of Methylated DNA using Capillary Electrophoresis: Fragment Analysis, Direct Sequencing, and Troubleshooting Sequencing of Bisulfite-Converted Genomic DNA; Environmental Influences on DNA Methylation; DNA Methylation in Zebrafish; Epigenetic Reprogramming and Developmental Potentials of Cloned Embryos after Somatic Cell Nuclear Transfer; Genomic-wide DNA Methylation Profiling Methods- From RLGS to Miami; Epigenetic Regulation of the Genome during Neurodevelopment and in Disease; Cell-Cycle Dependent Methylation of mtDNA in the framework of the Cascade of Methyl Groups involving nDNA, RNA and Proteins; In search of Asymmetric Cytosine Methylation in Exons 5-8 fo p53; Index.NER(01): GB IE
