基本説明
Reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current applications, methods of production, and quality control.
Full Description
In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism's genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Medicine series, these chapters contain detailed practical procedures on the latest DNA vaccine technology. The enthusiasm for DNA vaccine technology is made clear by the number of research studies published on this topic since the mid-1990s.
Contents
DNA Vaccine Design.- DNA Vaccine Design.- Design of Plasmid DNA Constructs for Vaccines.- Vaccination With Messenger RNA.- A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines.- In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors.- DNA Vaccine Delivery Systems.- Delivery of DNA Vaccines Using Electroporation.- Needle-Free Injection of DNA Vaccines: A Brief Overview and Methodology.- Needle-Free Delivery of Veterinary DNA Vaccines.- Surface-Modified Biodegradable Microspheres for DNA Vaccine Delivery.- A Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Nonviral Hybrid Approach.- Identification of Compartments Involved in Mammalian Subcellular Trafficking Pathways by Indirect Immunofluorescence.- DNA Vaccine Adjuvants and Activity Enhancement.- Adjuvant Properties of CpG Oligonucleotides in Primates.- Complexes of DNA Vaccines With Cationic, Antigenic Peptides Are Potent, Polyvalent CD8+ T-Cell-Stimulating Immunogens.- Prime-Boost Strategies in DNA Vaccines.- Modifying Professional Antigen-Presenting Cells to Enhance DNA Vaccine Potency.- Replicase-Based DNA Vaccines for Allergy Treatment.- DNA Vaccine Applications.- Immunological Responses of Neonates and Infants to DNA Vaccines.- DNA Vaccines for Allergy Treatment.- Protection From Autoimmunity by DNA Vaccination Against T-Cell Receptor.- The Use of Bone Marrow-Chimeric Mice in Elucidating Immune Mechanisms.- DNA Vaccine Production, Purification, and Quality.- A Simple Method for the Production of Plasmid DNA in Bioreactors.- Practical Methods for Supercoiled pDNA Production.- Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines.- Large-Scale, Nonchromatographic Purification of Plasmid DNA.- Assuring the Quality, Safety, and Efficacy of DNAVaccines.
