Full Description
This book provides the immune oncology (IO) community with a deeper understanding of the scope of the biomarker methods to potentially improve the outcome from immunotherapy. The editors secured the input from experts in the field dedicated to translating scientific research from bench to bedside was submitted. The book provides not only details about the technical, standardization and interpretation aspects of the methods but also introduces the reader to the background information and scientific justification for selected biomarkers and assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Contents
Status of Immune-oncology: Challenges and Opportunities.- Immunological Targets for Immunotherapy: Inhibitory T Cell Receptors.- Measuring Tumor Mutational Burden Using Whole Exome Sequencing.- Germline Genetics in Immuno-Oncology: From Genome-wide to Targeted Biomarker Strategies.- Detection of Microsatellite Instability Biomarkers via Next-generation Sequencing.- Genetic Instability Markers in Cancer.- Validation of Circulating Tumor DNA Assays for Detection of Metastatic Melanoma.- Techniques Associated with Exosome Isolation for Biomarker Development - Liquid Biopsies for Ovarian Cancer Detection.- Identifying MicroRNA Pathway Variants as Biomarkers of Patient Selection for Immune Therapy.- Histone Modifications as Biomarkers for Immunotherapy.- Functional Genome Profiling to Understand Cancer Immune Responsiveness.- Using RNA-sequencing to Characterize the Tumor Microenvironment.- Development of Gene Expression Based Biomarkers on the nCounter® Platform for Immuno-Oncology Applications.- The CRISPR System and Cancer Immunotherapy Biomarkers.- HLA Class I Antigen Processing Machinery Defects in Cancer Cells - Frequency, Functional Significance and Clinical Relevance with Special Emphasis on their Role in T cell-based Immunotherapy of Malignant Disease.- High-parameter Immune Profiling with CyTOF.- The Adaptome as Biomarker for Assessing Cancer Immunity and Immunotherapy.- Single Molecule Arrays for Ultrasensitive Detection of Blood-based Biomarkers for Immunotherapy.- Single-cell Multiplexed Proteomics on the IsoLight Resolves Cellular Functional Heterogeneity to Reveal Clinical Responses of Cancer Patients to Immunotherapies.- Methods to Detect Immunogenic Cell Death in vivo.- Overview of Tissue Imaging Methods.- Multiplex Immunofluorescence Assays.- Multiplexed Immunohistochemical Consecutive Staining on Single Slide (MICSSS): Multiplexed Chromogenic IHC Assay for High-Dimensional Tissue Analysis.- Cyclic Multiplexed-Immunofluorescence (cmIF),A Highly Multiplexed Method for Single-cell Analysis.- High-Plex Spatially Resolved RNA and Protein Detection using Digital Spatial Profiling: A Technology Designed for Immuno-Oncology Biomarker Discovery and Translational Research.- The InSituPlex® Staining Method for Multiplexed Immunofluorescence Cell Phenotyping and Spatial Profiling of Tumor FFPE Samples.- Microbiome as an Immunological Modifier.- Systems Biology for Multi-Platform Data Integration - Overview.- Bioinformatics Tools and Resources for Cancer Immunotherapy Study.- Multiplatform Integrative Analysis of Immunogenomic Data for Biomarker Discovery.- Translating Immuno-Oncology Biomarkers to Diagnostic tests: A regulatory Perspective.
