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Full Description
The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource forall researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.
Contents
Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains.- Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed in planta.- Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeA.- A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions.- Quantification of Extracellular ATP in Plant Suspension Cell Cultures.- Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen Interactions.- Method to Study Dynamics of Membrane-Bound Plant Transcription Factors during Biotic Interactions in Tomato.- Preparation of Plant Material for Analysis of Protein-Nucleic Acid Interactions by FRET-FLIM.- Analysis of DNA Methylation Profile in Plants by Chop-PCR.- Change in Nucleosome Dynamicsduring Stress Responses in Plants.- Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry.- A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light Environments.- Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant Pathogen Pseudomonas syringae.- Quantification of Cauline Leaf Abscission in Response to Plant Pathogens.- Methods for Replicating Leaf Vibrations Induced by Insect Herbivores.- Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes.- The Apple Fruitlet Model System for Fire Blight Disease.- Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R Proteins.- Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems.- Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants.- Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth.- A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance.
