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Full Description
This volume explores experimental strategies to study progranulin as a regulatory protein in biological and disease processes. The chapters in this book are divided into four parts: Part One is an introduction to the topic; Part Two looks at analytical and in vitro methods to study progranulin biology; Part Three discusses the role of progranulin in cancer; and Part Four describes the process of using animal models to study progranulin in health and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Progranulin: Methods and Protocols is a valuable resource for experienced researchers who want to expand their studies of this field, and newcomers who want to begin including progranulin into their work.
Contents
A Brief Overview of Progranulin in Health and Disease.- Chromatographic Methods for the Purification of Granulin Peptides.- Methods for Expression and Purification of Biologically Active Recombinant Progranulin.- Large-Scale Generation of Recombinant Granulin Peptides in E. coli.- Nuclear Magnetic Resonance Spectroscopy in Analysis of Granulin Three-Dimensional Structure and Cysteine Bridging.- Data Mining: Applying the AD&FTD Mutation Database to Progranulin.- Measurement of Circulating Progranulin (PGRN/GP88/GEP) by Enzyme-Linked Immunosorbent Assay and Application in Human Diseases.- Immunohistochemical Detection of Progranulin (PGRN/GP88/GEP) in Tumor Tissues as a Cancer Prognostic Biomarker.- Analysis of Progranulin-Mediated Akt and MAPK Activation.- Mouse Monoclonal Antibodies Against Progranulin (PGRN/GEP) as Therapeutics in Preclinical Cancer Models.- Methods to Analyze the Role of Progranulin (PGRN/GEP) on Cancer Stem Cell Features.- Methods to Study the Role of Progranulin in the Tumor Microenvironment.- Methods to Investigate the Molecular Basis of Progranulin Action on Neurons In Vivo using Caenorhabditis elegans.- The Use of Caenorhabditis elegans to Study Progranulin in the Regulation of Programmed Cell Death and Stress Response.- Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function.- Methods to Investigate the Molecular Basis of Progranulin Actions on Brain and Behavior In Vivo Using Knockout Mice.- Methods to Investigate the Protection Againts Neurodegenerative Disorders Provided by Progranulin Gene Transfer in the Brain.- The Interaction between Progranulin with Sortilin and the Lysosome.- Methods to Study the Role of Progranulin in Preimplanatation Mouse Embryo Development.- Establishment of a Modified Collagen-Induced Arthritis Mouse Model to Investigate the Anti-Inflammatory Activity of Progranulinin Inflammatory Arthritis.- Methods for Studying the Function of Progranulin in Atherosclerosis using Both Knockout Mice Models and In Vitro Studies.- Methods to Investigate the Roles of Prgranulin in Angiogenesis Using In Vitro Strategies and Transgenic Mouse Models.
