Full Description
This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs. Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interestedin learning more about reporter genes.
Contents
Genome-Edited Cell Lines for High-Throughput Screening.- High-Throughput Firefly Luciferase Reporter Assays.- Using the 2A Protein Co-Expression System: Multi-Cistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins.- Developing Mammalian Cellular Clock Models Using Firefly Luciferase Reporter.- High-Throughput Screening Method to Identify Alternative Splicing Regulators.- High-Throughput Screening of a Luciferase Reporter of Gene Silencing on the Inactive X Chromosome.- Making It All Work: Functional Genomics and Reporter Gene Assays.- Reporter Gene Assays Using Transfectable Functional Genomics Libraries.- Reporter Gene Assays Using Viral Functional Genomics Libraries.- Using YFP as a Reporter of Gene Expression in the Green Alga Chlamysomonas reinhardtii.- Gene Expression Analysis by Arylsulfatase Assays in the Green Algo Chlamydomonas reinhardtii.- Endogenous Locus Reporter Assays.- High Content Reporter Assays.- Data Mining and Computational Modeling of High-Throughput Screening Datasets.- Intravital Imaging by Two-Photon Excitation Microscopy of Human Melanoma Cells in the Mouse Ear Skin.
