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Full Description
This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field.
Contents
Metabolic Profiling: Topic, Challenges, Perspective.- Quality Control and Validation Issues in LC-MS Metabolomics.- Data Treatment for LC-MS Untargeted Analysis.- Bio- and Chemoinformatics Approaches for Metabolomics Data Analysis.- HILIC- MS/MS Multi-Targeted Method for Metabolomics Applications.- Ion Pair Chromatography for Endogenous Metabolites LC-MS Analysis in Tissue Samples Following Targeted Acquisition.- LC-MS Untargeted Analysis.- NMR Based Metabolic Profiling Procedures for Biofluids, and Cell and Tissue Extracts.- Untargeted GC-MS Metabolomics.- Rat Fecal Metabolomics Based Analysis.- GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol.- Sheathless Capillary Electrophoresis-Mass Spectrometry for the Profiling of Charged Metabolites in Biological Samples.- Two-Phase Extraction for Comprehensive Analysis of the Plant Metabolome by NMR.- NMR Spectroscopy Protocolsfor Food Metabolomics Applications.- Direct Injection Analysis of Fruit VOCs by PTR-ToF-MS: The Apple Case Study.- LC-MS Untargeted Protocol for the Analysis of Wine.- Tissue Multiplatform-Based Metabolomics/Metabonomics for Enhanced Metabolome Coverage.- UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics).- Metabolomics in Human Acute-Exercise Trials: Study Design and Preparation.
