Full Description
This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of study. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Glutamate Receptors: Methods and Protocols covers well-established glutamate receptor techniques, as well as new and cutting-edge techniques designed so that students, researchers, and clinicians can gain an understanding of the issues surrounding each experimental technique and its potential applications.
Contents
Single Molecule FRET Methods to Study Glutamate Receptors.- Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM).- Considerations for Imaging and Analyzing Neural Structures by STED Microscopy.- Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors.- Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function.- Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE).- Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus.- Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats.- Whole-Cell Patch Clamp Electrophysiology to Study Inotropic Glutamatergic Receptors and Their Roles in Addiction.- Gene Expression Analysis by Multiplex Single Cell RT-PCR.- Metabolomics Analysis of Glutamate Receptor Function.- Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation.- Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Function.- Fractionation of Subcellular Compartments from Human Brain Tissue.- Glutamate Receptor Antibodies in Autoimmune Central Nervous System Disease: Basic Mechanisms, Clinical Features, and Antibody Detection.
