Full Description
This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue models; assessment of ovarian granulosa cells with exposure to nanoparticles; DNA methylation analysis; evaluation of genotoxicity of nanoparticles in mouse models; and studying nanotoxicity using embryonic zebrafish. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Nanotoxicity: Methods and Protocols is a valuable resource for researchers and postgraduate students in the fields of toxicology, environmental science, pharmacological sciences, and clinical medicine. This volume also serves as a starting point for researchers interested in learning more about this developing field.
Contents
In Vitro Methods for Assessing Nanoparticle Toxicity.- Methods for Assessing Mast Cell Responses to Engineered Nanomaterial Exposure.- Preparation and Utilization of a 3D Human Liver Microtissue Model for Nanotoxicological Assessment.- Deriving Neural Cells from Pluripotent Stem Cells for Nanotoxicity Testing.- Methods for Evaluation of Ovarian Granulosa Cells with Exposure to Nanoparticles.- In Vitro Approaches for Assessing the Genotoxicity of Nanomaterials.- Measurement of Nanoparticle Induced Mitochondrial Membrane Potential Alterations.- Application of Gelatin Zymography in Nanotoxicity Research.- Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies.- Analysis of Nanomaterial Toxicity by Western Blot.- Quantification of Mitochondrial Genome Transcription in Male Reproductive Cells by Real-Time PCR.- DNA Methylation Analysis.- Evaluation of Nrf2 with Exposure to Nanoparticles.- Application of Electron Microscopes in Nanotoxicity Assessment.- Metabolic Fingerprinting of Bacteria Exposed to Nanomaterials, Using Online Databases, NMR, and High-Resolution Mass Spectrometry.- Methods to Quantify Nanomaterial Association with, and Distribution Across, the Blood-Brain Barrier In Vivo.- Evaluation of Genotoxicity of Nanoparticles in Mouse Models.- Evaluation of Pulmonary Toxicity of Nanoparticles by Bronchoalveolar Lavage.- Pulmonary Function Testing in Animals.- Nanotoxicity Assessment Using Embryonic Zebrafish.- Application of Daphnia magna for Nanoecotoxicity Study.- The Overview of Methods of Nanoparticle Exposure Assessment.
