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Full Description
In this volume, expert researchers in the field detail the most up-to-date methods commonly used to study and produce carotenoids. These include methods on the manipulation and metabolic engineering of carotenoid producing microalgae and bacteria, including Corynebacterium glutamicum, Rhodopseudomonas palustris and radio-tolerant bacteria; in addition to fungi, as the beta-carotene producing Blakeslea trispora and Mucor circinelloides or the lycopene producing Blakeslea trispora; and the heterobasidiomycetous yeast producing xanthophylls Xanthophyllomyces dendrorhous (Phaffia rhodozyma) and the engineered yeast Pichia pastoris. Additionally, three overview chapters on the advancement of Biotechnology and carotenoid production are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Microbial Carotenoids: Methods and Protocols provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms, aiming to ensure successful results in the further study of this vital field.
Contents
Advancement of Biotechnology by Genetic Modifications.- Carotenoids Production: A Healthy and Profitable Industry.- Carotenoids: From Plants to Food and Feed Industries.- Express Analysis of Microalgal Secondary Carotenoids by TLC and UV-Vis Spectroscopy.- Batch Cultivation for Astaxanthin Analysis Using the Green Microalga Chlorella zofingiensis under Multitrophic Growth Conditions.- Preparative Recovery of Carotenoids from Microalgal Biomass.- Adaptive Laboratory Evolution for Enhanced Carotenoid Production in Microalgae.- Carotenoid Production by Recombinant Corynebacterium glutamicum: Strain Construction, Cultivation, Extraction and Quantification of Carotenoids and Terpenes.- Rapid and Selective Screening Method for Isolation and Identification of Carotenoid-producing Bacteria.- Purification and Identification of Astaxanthin and its Novel Derivative Produced by Radio-tolerant Sphingomonas astaxanthinifaciens.- Screening, Isolation, and Identification of Zeaxanthin-producing Bacteria.- Synthesis of Carotenoids of Industrial Interest in the Photosynthetic Bacterium Rhodopseudomonas palustris: Bioengineering and Growth Conditions.- Molecular Tools for Carotenogenesis Analysis in the Mucoral Mucor circinelloides.- Expression Vectors and Gene Fusions for the Directed Modification of the Carotenoid Biosynthesis Pathway in Mucor circinelloides.- Lycopene Production by Mated Fermentation of Blakeslea trispora.- HPLC Analysis of Carotenoids in Neurosporaxanthin-producing Fungi.- Extraction and Analysis of Carotenes and Xanthophylls Produced by Xanthophyllomyces dendrorhous.- Isolation and Selection of New Astaxanthin Producing Strains of Phaffia rhodozyma.- Engineering Pichia pastoris for the Production of Carotenoids.- Isolation and Characterization of Extrachromosomal Double-Stranded RNA Elements from Carotenogenic Yeasts.
