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Full Description
This detailed volume explores numerous protocols that can be specifically used for studying plant programmed cell death (PCD), a mechanism involved in a number of physiological and pathological processes that are triggered by developmental requirements as well as changing/adverse environmental conditions. Rich in methodological recommendations, this book enables readers to perform the described analyses, alerting them to possible difficulties and showing how the methods can also be applied in different contexts and/or on different plant matrices. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Programmed Cell Death: Methods and Protocols provides essential methodological information that will help researchers to expand the field of plant PCD studies.
Contents
Programmed Cell Death in Plants: An Overview.- The Investigation of Morphological Features of Autophagy During Plant Programmed Cell Death.- Markers of Developmentally-Regulated Programmed Cell Death and Their Analysis in Cereal Seeds.- Measurement of Hypersensitive Cell Death Triggered by Avirulent Bacterial Pathogens in Arabidopsis.- Immunity-Associated Programmed Cell Death as a Tool for the Identification of Genes Essential for Plant Innate Immunity.- Analysis of Mitochondrial Markers of Programmed Cell Death.- Studying Retrograde Signaling in Plants.- Reactive Oxygen Species and Cell Death Detection in Tomato Plants Infected by Root Knot Nematodes.- Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death.- DNA Diffusion Assay Applied to Plant Cells.- Analysis of Reactive Carbonyl Species Generated Under Oxidative Stress.- In Vivo Analysis of Calcium Levels and Glutathione Redox Status in Arabidopsis Epidermal Leaf Cells Infected with the Hypersensitive Response-Inducing Bacteria Pseudomonas syringae pv. tomato AvrB (PstAvrB).- Measurement of Cyclic GMP During Plant Hypersensitive Disease Resistance Response.- Detection of MAPK3/6 Phosphorylation During Hypersensitive Response (HR)-Associated Programmed Cell Death in Plants.- Measurement of the Caspase-1-Like Activity of Vacuolar Processing Enzyme in Plants.- Plant Cell Cultures as Model Systems to Study Programmed Cell Death.
