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Full Description
This detailed volume explores numerous multidisciplinary approaches employed to analyze the role of different molecules or strategies used by different guests to survive inside hosts. Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Host-Pathogen Interactions: Methods and Protocols provides many molecular and bioinformatics techniques currently in use at reputed laboratories in countries all around the world, vital for many kinds of host-pathogen interactions studies.
Contents
Genetic Association Studies in Host-Pathogens Interaction Analysis.- Bacterial Genotyping Methods: From the Basics to Modern.- Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.- The Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection.- lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.- Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity.- Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage.- Organoids as a Model to Study Infectious Disease.- Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins.- Systems Biology Modeling to Study Pathogen-Host Interactions.- Phage Therapy: Various Perspectives on How to Improve the Art.- Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression.- Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth.- Detection of Bacterial Quorum Sensing Molecules.- Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae.- Introduction of Genetic Material in Ralstonia solanacearum through Natural Transformation and Conjugation.- In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors.- Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains.- Methods to Quantify Biotic-Induced Stress in Plants.- From Sample to Data: Preparing, Obtaining, and Analyzing Images of Plant-Pathogen Interactions Using Confocal Microscopy.- Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria.- Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria.- A New, Non-Destructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean.- Methods for the Characterization of Plant-Growth Promoting Rhizobacteria.
