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Full Description
This volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Lysosome: Methods and Protocols aims to provided protocols that will guide and inspire further research and generate new insights into this fascinating organelle.
Contents
1. SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS Melanie Thelen, Dominic Winter, Thomas Braulke, and Volkmar Gieselmann2. Quantitative Profiling of Lysosomal Lipidome by Shotgun LipidomicsMesut Bilgin, Jesper Nylandsted, Marja Jaattela, and Kenji Maeda3. Analysis of N- And O-Glycosylation of Lysosomal GlycoproteinsElmira Tokhtaeva, Olga A. Mareninova, Anna S. Gukovskaya, and Olga Vagin4. Analyzing Lysosome Related Organelles By Electron MicroscopyIlse Hurbain, Maryse Romao, Ptissam Bergam, Xavier Heiligenstein, And Graca Raposo 5. Microscopic Analysis of Lysosomal Membrane Permeabilization Ana Maria Vilamill Giraldo, Karin OEllinger, and Vesa Loitto6. Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets Frederik W. Lund and Daniel Wustner7. Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and TissuesMingguang Ren, Beibei Deng, Xiuqi Kong, Yonghe Tang, and Weiying Lin8. Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent RecoveryTakanobu Otomo and Tamotsu Yoshimori9. Delivery of Cargo to Lysosomes using GneosomesKristina M. Hamill, Ezequiel Wexselblatt, Wenyong Tong, Jeffrey D. Esko, and Yitzhak Tor10. Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles Camilla Loeoev and Anna Erlandsson 11. Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded CellsIda Eriksson, Karin OEllinger, and Hanna Appelqvist 12. Detection of Lysosomal Exocytosis in Platelets by Flow CytometryAnna L. Soedergren and Sofia Ramstroem13. Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal EpitopesNorma W. Andrews14. Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and SubstratesNeil D. Rawlings15. Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of LysoplexGiuseppina Di Fruscio, Sandro Banfi, Vincenzo Nigro, and Andrea Ballabio16. Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L FormsYoko Hashimoto17. Methods for Determination of -Glycosidase, -Glycosidase, and -Galactosidase Activities in Dried Blood Spot SamplesEser Yildirim Sozmen and Ebru Demirel Sezer18. Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic VilliJyotsna Vermaa, Sunita Bijarnia-Mahaya, and Ishwar C. Vermaa19. Lysosomal Biology in Cancer Colin Fennelly and Ravi Amaravadi
