Full Description
This second edition provides updated and new chapters on T-Cell trafficking. In addition to detailed experimental procedures, the interested reader will find informative introductory chapters on the relevance of T-Cell trafficking in thymic population and maturation, traffic through secondary lymphoid organs during 'physiological' resolving inflammation and during immune responses, as well as T-Cell trafficking in chronic inflammatory diseases. Importantly, chapters cover methods from in silico modeling of cellular interactions, in vitro adhesion assays, through ex vivo functional assays to integrated intravital modeling of T-Cell trafficking through organs. Written in the highly successful Methods in Molecular Biology series format, each methods chapter includes a short introduction to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, T-Cell Trafficking: Methods and Protocols, Second Edition aims to be an essential point of reference for those new to the field of T-Cell trafficking, or to those looking to expand their technical capabilities.
Contents
Introduction to Homeostatic Migration.- Analysis of Thymocyte Migration, Cellular Interactions, and Activation by Multi-Photon Fluorescence Microscopy of Live Thymic Slices.- Visualising and Tracking T Cell Motility in vivo.- Graph Theory-based Analysis of the Lymph Node Fibroblastic Reticular Cell Network.- Introduction: T cell Trafficking in Inflammation and Immunity.- Leukocyte Adhesion under Haemodynamic Flow Conditions.- Endocrine Regulation of Lymphocyte Trafficking In Vitro.- Mesenchymal Stromal Cells as Active Regulators of Lymphocyte Recruitment to Blood Vascular Endothelial Cells.- Monitoring RhoGTPase Activity in Leukocytes using Classic "pull-down" Assays.- Utilising Lentiviral Gene Transfer in Primary Endothelial Cells to Assess Lymphocyte-Endothelial Interactions.- Introduction to Lymphocyte Trafficking in Disease.- Using Ex-Vivo Liver Organ Cultures to Measure Lymphocyte Trafficking.- In vitro and Ex Vivo Models to Study T Cell Migration through the Human Liver Parenchyma.- Monitoring Migration of Activated T Cells to Antigen-rich Non-lymphoid Tissue.- Tissue Digestion for Stromal Cell and Leukocyte Isolation.- T-cell Response in the Lung Following Influenza Virus Infection.
