Full Description
This volume provides all the essential protocols that are currently used to study the immune synapse. Chapters in The Immune Synapse: Methods and Protocols cover methods for the study of the dynamics of immune synapse assembly, traffic at the immune synapse, new high resolution imaging, biophysical and computational methods for the study of the immune synapse, effector immune synapses, B cell, NK and mast cell immune synapses, and immune interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Immune Synapse: Methods and Protocols will be of interest to immunologists and, at a more general level, to cell biologists, biophysicists and computational biologists.
Contents
The Immune Synapse: Past, Present, and Future.- Analyzing Actin Dynamics of the Immunological Synapse.- Analysis of Microtubules and Microtubule-Organizing Centre at the Immune Synapse.- Analyzing the Dynamics of Signaling Microclusters.- Reconstitution of TCR Signaling using Supported Lipid Bilayers.- Plasma Membrane Sheets for Studies of B Cell Antigen Internalization From Immune Synapses.- Studying The Dynamics Of TCR Internalization At The Immune Synapse.- T Cell Receptor Activation of NF-Κb in Effector T Cells: Visualizing Signaling Events Within and Beyond the Cytoplasmic Domain of the Immunological Synapse.- Imaging Vesicular Traffic at the Immune Synapse.- Analysis of TCR Recycling at the Immune Synapse.- Simultaneous Membrane Capacitance Measurements and TIRF Microscopy to Study Granule Trafficking at Immune Synapses.- Mathematical Modeling of Synaptic Patterns.- Super-Resolution Analysis of TCR-Dependent Signaling: Single Molecule Localization Microscopy.- Förster Resonance Energy Transfer to Study TCR-pMHC Interactions in the Immunological Synapse.- Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes.- Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains during Immunological Synapse Formation using Donor-Quenching FRET and FLIM-FRET.- Revealing the Role of Microscale Architecture in Immune Synapse Function through Surface Micropatterning.- Spatial Control of Biological Ligands on Surfaces.- Probing Synaptic Biomechanics using Micropillar Arrays.- Microchannels for Study of Immunological Synapses and Kinapses.- Purification of LAT Containing Membranes from Resting and Activated T Lymphocytes.- Quantitative Phosphoproteomic Analysis of T-Cell Receptor Signaling.- Imaging Asymmetric T Cell Division.- Ultrastructure Of Immune Synapses.- Systems Imaging of the Immune Synapse.- Comprehensive Analysis of Immunological Synapse Phenotypes using Supported Lipid Bilayers.- Studying Immunoreceptor Signaling in Human T Cells using Electroporation of In Vitro Transcribed mRNA.- A Protein Expression Tool-Kit for Studying Signalling in T Cells.- Imaging The Effector CD8 Synapse.- The Mast Cell Antibody-Dependent Degranulatory Synapse.- Measurement of Lytic Granule Convergence after Formation of an NK Cell Immunological Synapse.- Studying the T Cell-Astrocyte Immune Synapse.- Aberrant Immunological Synapses Driven by Leukemic Antigen Presenting Cells.- Studying the Immune Synapse in HIV-1 Infection.- In Vivo Imaging of T Cell Immunological Synapses and Kinapses in Lymph Nodes.- Studying Dendritic Cell-T Cell Interactions Under In Vivo Conditions.
