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Full Description
This second edition volume provides an overview of some of the types of nanostructures commonly used in nanobiomedicine. The chapters in this book discuss practical information on the synthesis and characterization of a variety of solution-phase and surface-bound nanomaterials, with examples of how they can be used in sensing, imaging, and therapeutics. Specific topics include the synthesis and characterization of molecule and biomolecule-functionalized nanoconjugates with gold, iron oxide, or polymeric cores; the development of biosensing, imaging, and therapeutic applications of multicomponent/multifunctional nanostructures; and the application of flow cytometry in nanobiomedicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.<
Thorough and comprehensive, Biomedical Nanotechnology: Methods and Protocols, Second Edition is a useful resource for scientists and researchers at all levels who are interested in working in a new area of nanoscience and technology, or in expanding their knowledge base in their current field.
Contents
Quantification of siRNA Duplexes Bound to Gold Nanoparticle Surfaces.- Ligand Exchange and 1H NMR Quantification of Single- and Mixed-Moiety Thiolated Ligand Shells on Gold Nanoparticles.- Nanoparticle Tracking Analysis for Determination of Hydrodynamic Diameter, Concentration, and Zeta Potential of Polyplex Nanoparticles.- Magnetic Characterization of Iron Oxide Nanoparticles for Biomedical Applications.- Preparation of Magnetic Nanoparticles for Biomedical Applications.- Brain-Penetrating Nanoparticles for Analysis of the Brain Microenvironment.- Volumetric Bar-Chart Chips for Biosensing.- qFlow Cytometry-Based Receptoromic Screening: A High-Throughput Quantification Approach Informing Biomarker Selection and Nanosensor Development.- Evaluating Nanoparticle Binding to Blood Compartment Immune Cells in High-Throughput with Flow Cytometry.- A Gold@Polydopamine Core-Shell Nanoprobe for Long-Term Intracellular Detection of MicroRNAs in Differentiating Stem Cells.- Antibody-Conjugated Single Quantum Dot Tracking of Membrane Neurotransmitter Transporters in Primary Neuronal Cultures.- Spectroscopic Photoacoustic Imaging of Gold Nanorods.- Dual Wavelength-Triggered Gold Nanorods for Anti-Cancer Treatment.- Photolabile Self-Immolative DNA-Drug Nanostructures.- Enzyme-Responsive Nanoparticles for the Treatment of Disease.- NanoScript: A Versatile Nanoparticle-Based Synthetic Transcription Factor for Innovative Gene Manipulation.- Glucose-Responsive Insulin Delivery by Microneedle-Array Patches Loaded with Hypoxia-Sensitive Vesicles.- Electrospun Nanofiber Scaffolds and their Hydrogel Composites for the Engineering and Regeneration of Soft Tissues.- Application of Hydrogel Template Strategy in Ocular Drug Delivery.- High-Accuracy Determination of Cytotoxic Responses from Graphene Oxide Exposure using Imaging Flow Cytometry.- Air-Liquid Interface Cell Exposures to Nanoparticle Aerosols.- Returning to the Patent Landscapes for Nanotechnology: Assessing the Garden That It Has Grown Into.
