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Full Description
This volume provides a unique forum to review cell microencapsulation in a broad sense by exploring various cell types that have been encapsulated for different purposes, different approaches and devices used for microencapsulation, the biomaterials used in cell microencapsulation, the challenges to the technology, and the current status of its application in different clinical situations. This book is divided in five sections: Section I is an introductory part that discusses historical developments of the technology and its current challenges, as well as the various applications of cell microencapsulation; Section II discusses the main approaches and devices currently used in cell microencapsulation; Section III presents an overview of the various polymeric materials currently in use for cell microencapsulation and the enabling technologies to either monitor or enhance encapsulated cell function; Section IV gives specific examples of the methods used to encapsulate various cell types;and Section V provides an overview of the different clinical situations in which cell microencapsulation has been applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Cell Microencapsulation: Methods and Protocols is a valuable reference for researchers, engineers, clinicians, and other healthcare professionals, as well as food technologists who will find detailed descriptions of methods for the microencapsulation of specific cell types and their current of potential clinical and industrial applications. This volume also includes detailed information about the design and manufacture of different devices including large-scale production devices for use in cell microencapsulation.
Contents
Historical Perspectives and Current Challenges in Cell Microencapsulation.- Applications of Cell Microencapsulation.- Cell Microencapsulation: Dripping Methods.- Field Effect Microparticle Generation for Cell Microencapsulation.- Microfluidic Approach to Cell Microencapsulation.- Polymeric Materials for Cell Microencapsulation.- Polymeric Materials for Perm-Selective Coating of Alginate Microbeads.- Determination of the Mechanical Strength of Microcapsules.- The Diffusive Properties of Hydrogel Microcapsules for Cell Encapsulation.- Methods for Incorporating Oxygen Generating Biomaterials into Cell Culture and Microcapsule Systems.- Non-Invasive Tracking of Alginate-Microencapsulated Cells.- Retrieval of Microencapsulated Islet Grafts for Post-Transplant Evaluation.- A Method of Porcine Pancreatic Islet Isolation for Microencapsulation.- Selective Osmotic Shock (SOS)-Based Islet Isolation for Microencapsulation.- Preparation and Characterization of Alginate-Chitosan Microcapsule for Hepatocyte Culture.- Use of Flow Focusing Technique for Microencapsulation of Myoblasts.- Alginate Microbeads for Cell and Protein Delivery.- Compartmentalization of Two Cell Types in Multi-Layered Alginate Microcapsules.- Primary Choroid Plexus Tissue for Use in Cellular Therapy.- Microencapsulation of Stem Cells for Therapy.- Microencapsulated Cells for Cancer Therapy.- Microencapsulation of Bacterial Cells by Emulsion Technique for Probiotic Application.- Microencapsulation of Islets for the Treatment of Type 1 Diabetes Mellitus (T1D).- Immunological Challenges Facing Translation of Alginate Encapsulated Porcine Islet Xenotransplantation to Human Clinical Trials.- Microencapsulation in Clinical Islet Xenotransplantation.- Methods for Microencapsulated Porcine Islet Production.- Microencapsulation of Parathyroid Cells for the Treatment of Hypoparathyroidism.
