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Full Description
This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell microarrays, printing techniques for live-cell microarrays, live-cell microarray application, microfluidic live-cell arrays, and advanced materials suitable for live-cell microarrays. Some of the topics covered are artificial micro-niche array with spatially structured biochemical cues; inkjet printing for biomedical application; fabrication of multi-electrode arrays for neurobiology applications; rapid prototyping of thermoplastic microfluidic devices; and oxygen-generating photocrosslinkable hydrogel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Cell-Based Microarrays: Methods and Protocols is a valuable resource for anyone interested in expanding their knowledge and research of cell-based microarrays.
Contents
Next Generation Live-Cell Microarray Technologies.- Cell-Based Microarrays Using Superhydrophobic Platforms Patterned with Wettable Regions.- 3D Plasma Nanotextured® Polymeric Surfaces for Protein or Antibody Arrays, Biomolecule, and Cell Patterning.- Single Cell Microarrays Fabricated by Microscale Plasma-Initiated Protein Patterning (µPIPP).- Artificial Micro-Niche Array with Spatially Structured Biochemical Cues.- Three-Dimensional Cell-Based Microarrays: Printing Pluripotent Stem Cells into 3-D Microenvironments.- Fabrication of Biomolecule Microarrays for Cell Immobilization using Automated Microcontact Printing.- Hydrogel Printing Based on UV-Induced Projection for Cell-Based Microarray Fabrication.- Inkjet Printing for Biomedical Applications.- High Throughput Screening of Senescence Markers in Hematopoietic Stem Cells Derived from Induced Pluripotent Stem Cells.- Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems.- Fabrication of Multi-Electrode Arrays for Neurobiology Applications.- Rapid Prototyping of Thermoplastic Microfluidic Devices.- Fabrication of Biomolecule Microarrays using Rapid Photochemical Surface Patterning in Thiol-ene Based Microfluidic Devices.- Fabrication and Operation of Microfluidic Hanging-Drop Networks.- A Microfluidic Droplet Array System for Cell-Based Drug Combination Screening.- Establishment of Microfluidic Spheroid Cultures for Biomedical Applications.- Capillary Pinning Assisted Patterning of Cell-Laden Hydrogel Microarrays in Microchips.- Oxygen-Generating Photocrosslinkable Hydrogel.- A Self-Assembled Anti-Fouling Nano-Biointerface for the Generation of Spheroids.
