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Full Description
This volume aims to provide an update on recent developments in protein secretion studies in plants versus yeast and mammalian systems. This book also discusses case studies that analyze the use of plant protein secretion using various tools and systems. The chapters in this book explore topics such as the study of Golgi-mediated protein traffic in plant cells; actin-based intracellular trafficking in pollen tubes; secretion system for identification of cargo proteins of vacuolar sorting receptors; isolation of the plant exocyst complex; and plant autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Plant Protein Secretion: Methods and Protocols is a valuable resource for researchers interested in furthering their studies in plant protein secretion."
Contents
An Overview of Protein Secretion in Yeast and Animal Cells.- An Overview of Protein Secretion in Plant Cells.- Bioinformatics Analysis of Protein Secretion in Plants.- Proteomics Analysis of Secreted Proteins from Cell Microenvironment.- Using Homology Modelling to Understand the Structural Basis of Specific Interaction of a Plant-Specific AtSar1a/AtSec23a Pair Involved in Protein ER Export.- Analysis of Golgi-Mediated Protein Traffic in Plant Cells.- Analysis of Membrane Protein Topology in the Plant Secretory Pathway.- Semi-Automatic Segmentation of Plant Golgi Stacks in Electron Tomograms Using 3dmod.- 3D Printing of Plant Golgi Stacks from their Electron Tomographic Models.- Transient Expression of Chimeric Fluorescent Reporter Proteins in Pollen Tubes to Study Protein Polar Secretion and Dynamics.- Analysis of Actin-Based Intracellular Trafficking in Pollen Tubes.- Analysis of Phragmoplast Kinetics during Plant Cytokinesis.- Immunofluorescence Analysis of Membrane-Associated Proteins for Clathrin-Mediated Endocytosis in Plant Root Cells.- In Vivo Interaction Studies by Measuring Förster Resonance Energy Transfer through Fluorescence Lifetime Imaging Microscopy (FRET/FLIM).- Analysis of Nanobody-Epitope Interactions in Living Cells via Quantitative Protein Transport Assays.- A Secretion System for Identification of Cargo Proteins of Vacuolar Sorting Receptors.- Identifying Novel Regulators of Vacuolar Trafficking by Combining Fluorescence Imaging-Based Forward Genetic Screening and In Vitro Pollen Germination.- Measuring Plant Protein Secretion.- Transient Secretory Enzyme Expression in Leaf Protoplasts to Characterize SNARE Functional Classes in Conventional and Unconventional Secretion.- The Organelle pH Estimate and Measurement in Plant Secretory Pathway.- Analysis of Exocyst-Positive Organelle (EXPO)-Mediated Unconventional Protein Secretion in Plant Cells.- Isolation of the Plant Exocyst Complex.- Using Microscopy Tools to Visualize Autophagosomal Structures in Plant Cells.- Analysis of Plant Autophagy.
