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Full Description
Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques. In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.
Contents
Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry.- Thin-Layer Chromatography of Phospholipids.- Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics.- Using 2D Crystals to Analyze the Structure of Membrane Proteins.- Crystallization of Membrane Proteins.- Molecular Dynamics Simulations of Membrane Proteins.- Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins.- Topology Determination of Untagged Membrane Proteins.- self-assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses.- The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane.- Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance.- Peptide Interaction with and Insertion into Membranes.- Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems.- Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding Yeast.- Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins.- Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry.- Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondria.- Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells.- An Assay to Monitor the Membrane Integration of Single-Span Proteins.- Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria.- Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes.- Single Channel Analysis of MembraneProteins in Artificial Bilayer Membranes.- Quantification of Protein Complexes by Blue Native Electrophoresis.- Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions.
