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Full Description
Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future. In addition, considering the recent unparalleled progress of the "omics" tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Arabidopsis thaliana is acknowledged as the most important plant model system by the scientific community and Arabidopsis research has fundamentally influenced our understanding of the basic biology and ecology of plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Arabidopsis Protocols, Third Edition seeks to serve both experienced researchers and beginners with its detailed methodologies on this burgeoning scientific field.
Contents
Handling Arabidopsis Plants: Growth, Preservation of Seeds, Transformation and Genetic Crosses.- Using Arabidopsis Related Model Species (ARMS): Growth, Genetic Transformation and Comparative Genomics.- Growing Arabidopsis in vitro: Cell Suspensions, in vitro Culture, and Regeneration.- Arabidopsis Database and Stock Resources.- Bioinformatic Tools in Arabidopsis Research.- Exploiting Natural Variation in Arabidopsis.- Grafting in Arabidopsis.- Agrobacterium tumefaciens Mediated Transient Transformation of Arabidopsis thaliana Leaves.- iTilling: Personalized Mutation Screen.- Tailor Made Mutations in Arabidopsis Using Zinc-Finger Nucleases.- The Use of Artificial Micro RNA Technology to Control Gene Expression in Arabidopsis thaliana.- Generation and Identification of Arabidopsis EMS Mutants.- Generation and Identification of Arabidopsis T-DNA Insertion Mutants.- Identification of EMS-induced Causal Mutations in Arabidopsis thaliana by Next-Generation Sequencing.- Arabidopsis Transformation with Large Bacterial Artificial Chromosomes.- Global DNA Methylation Analysis Using Methyl-Sensitive Amplification Polymorphism (MSAP).- Next Generation Mapping of Genetic Mutations Using Bulk Population Sequencing.- Chemical Fingerprinting of Arabidopsis Using Fourier-transform Infrared (FT-IR) Spectroscopic Approaches.- A Pipeline for 15N Metabolic Labeling and Phosphoproteome Analysis in Arabidopsis thaliana.- Gene Expression Profiling Using DNA Microarrays.- Forward Chemical Genetic Screening.- Highly-Reproducible ChIP-On-Chip Analysis to Identify Genome-Wide Protein Binding and Chromatin Status in Arabidopsis thaliana.- Fluorescence Microscopy.- Immunocytochemical Fluorescent In Situ Visualization of Proteins in Arabidopsis.- High Pressure Freezing and Freeze Substitution of Arabidopsis For Electron Microscopy.- Applications of Fluorescent Marker Proteins in Plant Cell Biology.- Flow Cytometry and Sorting in Arabidopsis.- Live Imaging of Arabidopsis Development.- Arabidopsis Organelle Isolation and Characterisation.- Analysis of Subcellular Metabolite Distributions within Arabidopsis thaliana Leaf Tissue: A Primer for Subcellular Metabolomics.- Hormone Profiling.- Purification of Protein Complexes and Characterization of Protein-Protein Interactions.- Protein Fragment Bimolecular Fluorescence Complementation Analysis for the In vivo Study of Protein-Protein Interactions and Cellular Protein Complex Localizations.- The Split Ubiquitin System for the Analysis of Three-Component Interactions.- RNA-Binding Protein Immunoprecipitation from Whole Cell Extracts.- High Throughput Analysis of Protein-DNA Binding Affinity.
