Full Description
The de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider.
Contents
Building Block Synthesis using the Polymerase Chain Assembly (PCA) Method.-Oligonucleotide Assembly in Yeast to Produce Synthetic DNA Fragments.-TopDown Real-Time Gene Synthesis.-De novo DNA Synthesis using Single-molecule PCR.-SLIC: A Method for Sequence and Ligation Independent Cloning.-Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli.-Assembling DNA Fragments by USER Fusion.-Fusion PCR via Novel Overlap Sequences.-Using Recombineering to Generate Point Mutations: the Oligonucleotide-based "Hit & Fix" Method.-Using Recombineering to Generate Point Mutations: galK-Based Positive-Negative Selection Method.-Assembling Large DNA Segments in Yeast.-Recursive Construction of Perfect DNA Molecules and Libraries from Imperfect Oligonucleotides.-Cloning Whole Bacterial Genomes in Yeast.-Production of Infectious Poliovirus from Synthetic Viral Genomes.-In Silico Design of Functional DNA Constructs.-Using DNAWorks in Designing Oligonucleotides for PCR-Based Gene Synthesis.-De Novo Gene Synthesis Design Using TmPrime Software.-Design-A-Gene with GeneDesign.-Leading a Successful iGEM Team .-The Build-a-Genome Course.-DNA Synthesis Security.
