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Full Description
Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail. The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.
Contents
Introduction: Why Analyze Single Cells?.- Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview.- Identification of Enzyme-Converted Peptide Products from Single Cells Using Capillary Electrophoresis and Liquid Chromatography-Mass Spectrometry.- Laser Capture Microdissection for Gene Expression Analysis of Specific Cell Populations in Single Blastocysts.- Single-Cell Culture in Microwells.- Iso-Dielectric Separation and Analysis of Cells.- Single Cell Electroporation Using Microfluidic Devices.- Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Build-In Pillar Array.- Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay.- Droplet Microfluidics for Single-Cell Analysis.- Screening of Antigen-Specific Antibody Secreting Cells.- Analysis of Single Eukaryotic Cells Using Raman Tweezers.- Single-Cell Microinjection Technologies.- Ultrasonic Manipulation of Single Cells.- Expanding the Horizons for Single-Cell Applications on Lab-On-a-Chip Devices.- Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses.
