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Full Description
Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility. While significant advances in embryo culture have occurred over the last few decades. In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture. These include emerging methods and the impact of embryo culture on epigenetics and offspring health is presented to set the stage for future research and laboratory application involving embryo culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Embryo Culture: Methods and Protocols seeks to aid scientists in the further study of this crucially important field of embryology and assisted reproductive technologies.
Contents
Historical Background of Gamete and Embryo Culture.-Utility of Animal Models for Human Embryo Culture Development - Rodents.-Utility of Animal Models for Human Embryo Culture Development—Domestic Species.-Utility of Animal Models for Human Embryo Culture: Non-Human Primates.-Media Composition: Salts and Osmolality.-Media Composition: Energy Sources and Metabolism.-Media Composition - Amino Acids and Cellular Homeostasis.-Media Composition: Macromolecules and Embryo Growth.-Media Composition- Antioxidants/Chelators and Cellular Function.-Media Composition: pH and Buffers.-Media Composition, Growth Factors.-Culture Systems - Single Step.-Culture Systems - Sequential.-Embryo Coculture.-Low Oxygen Culture.-Culture Methods - Embryo Density.-Culture Medium - Air Quality.-Mineral Oil Overlay and Embryo Culture.-Physiological and Environmental Factors That Can Affect the Outcome of Human ART.-Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics.-Human Embryo Culture Media Comparisons.-Embryo Culture and Monozygotic Twinning.-Embryo Culture and Epigenetics.
