Full Description
Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.
Contents
Electroporation-Based Gene Therapy: Recent Evolution in the Mechanism Description and Technology Developments.- Electropermeabilization of the Cell Membrane.- The Impact of Non-Electrical Factors on Electrical Gene Transfer.- Electroporation Formulation for Cell Therapy.- Short-Fragment DNA-Mediated in Vivo DNA Electroporation Delivery.- Gold Nanoparticle-Enhanced Electroporation for Leukemia Cell Transfection.- SiRNA Delivery via Electropulsation: A Review of the Basic Processes.- Continuous Cell Electroporation for Efficient DNA and siRNA Delivery Based on Laminar Microfluidic Chips.- Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells.- Direct Imaging of siRNA Electrotransfer at the Single Cell Level.- Electroporation-Mediated siRNA Delivery into Tumors.- Substrate-Mediated, High Efficiency siRNA Electroporation.- Intradermal Electroporation of RNA.- Delivery of DNA into the Central Nervous System via Electroporation.- DNA Delivery in Adult Mouse Eyes: An Update with Corneal Outcomes.- Delivery of Plasmid DNA into Developing Rat Teeth by Electroporation.- Electroporation-Mediated Gene Delivery to the Lungs.- Electroporation-Mediated Delivery of Genes in Rodent Models of Lung Contusion.- In Vivo Electroporation-Mediated Gene Delivery to the Beating Heart.- Managing Local Swelling Following Intratumoral Electro-Chemo-Gene Therapy.- Gene Electrotransfer in Clinical Trials.- Electrochemotherapy in Veterinary Oncology: From Rescue to First-line Therapy.- Low Energy DC Current Ablation in a Mouse Tumor Model.- Evolution of Electroporated DNA Vaccines.- Clinical Development of Intramuscular Electroporation: Providing a "Boost" for DNA Vaccines.- Electroporation-Mediated Administration of Candidate DNA Vaccines against HIV-1.- Electroporation-Mediated Intradermal Delivery of DNA Vaccines in Nonhuman Primates.- Intramuscular DNA Vaccination Protocols Mediated by Electric Fields Pieranna.- DNA Electroporation ofMulti-Agent Vaccines Conferring Protection Against Select Agent Challenge—TriGrid Delivery System.- Gene-Based Vaccination and Screening Methods to Develop Monoclonal Antibodies.
