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Full Description
Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs. Updated chapters included in this edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model organisms, and medical genetics and drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and tips to troubleshoot and avoid known pitfalls.
Authoritative and cutting-edge, Bacterial Artificial Chromosomes, Second Edition seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies.
Contents
From Selective Full-Length Genes Isolation By TAR Cloning In Yeast To Their Expression From HAC Vectors In Human Cells.- Recombineering Linear BACs.- BAC Sequencing Using Pooled Methods.- Making BAC Transgene Constructs With Lambda-Red Recombineering System For Transgenic Animals Or Cell Lines.- Directing Enhancer-Traps And Itol2 End Sequences To Deleted BAC Ends With Loxp- and Lox511-Tn10 Transposons.- Recombining Overlapping Bacs Into Single Large Bacs.- Generation Of Knockout Alleles By RFLP Based BAC Targeting Of Polymorphic Embryonic Stem Cells.- Herpesvirus Mutagenesis Facilitated By Infectious Bacterial Artificial Chromosomes (Ibacs).- Conversion Of BAC Clones Into Binary BAC (BIBAC) Vectors and Their Delivery Into Basidiomycete Fungal Cells Using Agrobacterium tumefaciens.- Infectious Delivery of Alphaherpesvirus Bacterial Artificial Chromosomes.- A Recombineering-Based Gene Tagging System For Arabidopsis.- BAC Transgenic Zebrafish for Transcriptional Promoter and Enhancer Studies.- Bacs-On-BeadsTM (BobsTM) Assay for the Genetic Evaluation of Prenatal Samples And Products of Conception.- New BAC Probe Set To Narrow Down Chromosomal Breakpoints In Small and Large Derivative Chromosomes, Especially Suited for Mosaic Conditions.- BAC-Probes Applied For Characterization Of Fragile Sites (FS).- Application Of BAC-Probes To Visualize Copy Number Variants (CNVs).- Site-Specific Integration Of Bacterial Artificial Chromosomes Into Human Cells.- Generation Of BAC Reporter Cell Lines For Drug Discovery.
