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Full Description
The second edition of Plant Pathology: Techniques and Protocols covers diagnostic methods that are currently used in laboratories for a broad range of plant species and matrixes. These include serological and molecular methods that have one or more of the following characteristics: suitability for high-throughput testing, detection of a group of pathogens or of sometimes uncharacterized pathogens, detection and identification of specific pathogens, and high sensitivity. This volume discusses qualitative and quantitative tests, as well as recently developed diagnostic methods. It also provides background information on many pathogens, which are either endemic, non-endemic, or emerging and with different lifecycles that cause diseases of significant importance in a wide variety of hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, background information on pathogens and the disease caused, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Informative and cutting-edge, Plant Pathology: Techniques and Protocols, Second Edition is the perfect book for plant pathologists and molecular biologists who will use this information to test out the latest research in their laboratories.
Contents
Detection of the Bacterial Potato Pathogens Pectobacterium and Dickeya spp. Using Conventional and Real Time PCR.- Detection and Identification of Phoma Pathogens of Potato.- Diagnosis of Ramularia collo-cygni and Rhynchosporium spp in Barley.- A Real-Time Multiplex PCR Assay Used in the Identification of Closely Related Fungal Pathogens at the Species Level.- Diagnostics of Tree Diseases Caused by Phytophthora Species.- Real-Time LAMP for Chalara fraxinea Diagnosis.- Loop-Mediated Isothermal Amplification Procedure (LAMP) for Detection of the Potato Zebra Chip Pathogen 'Candidatus Liberibacter Solanacearum'.- Loop-Mediated Isothermal Amplification (LAMP) for Detection of Phytoplasmas in the Field.- Diagnosis of Phytoplasmas by Real-Time PCR Using Locked Nucleic Acid (LNA) Probes.- Q-Bank Phytoplasma: A DNA Bar-Coding Tool for Phytoplasma Identification.- High-Throughput Diagnosis of Potato Cyst Nematodes in Soil Samples.- Detection of Nepovirus Vector and Non-Vector Xiphinema Species in Grapevine.- Molecular and Serological Methods for the Diagnosis of Viruses in Potato Tubers.- Immunocapture-Multiplex RT-PCR for the Simultaneous Detection and Identification of Plant Viruses and Their Strains: Study Case, Potato Virus Y (PVY).- Snapshot and CE-SSCP: Two Simple and Cost-Effective Methods to Reveal Genetic Variability within a Virus Species.- Detection and Characterization of Viral Species/Sub-Species Using Isothermal Recombinase Polymerase Amplification (RPA) Assays.- Virus-Testing by PCR and RT-PCR Amplification in Berry Fruit.- Metagenomics Approaches Based on Virion-Associated Nucleic Acids (VANA): An Innovative Tool for Assessing without a Priori the Viral Diversity of Plants.- Detection of Potato spindle tuber viroid and Other Related Viroids by a DIG Labeled RNA Probe.- Microarray Platform for the Detection of a Range of Plant Viruses and Viroids.- Multiplex Detection of Plant Pathogens Throughthe Luminex Magplex Bead System.- Next Generation Sequencing of Elite Berry Germplasm and Data Analysis Using a Bioinformatics Pipeline for Virus Detection and Discovery.- Metagenomic Next-Generation Sequencing of Viruses Infecting Grapevines.- Droplet Digital PCR for Absolute Quantification of Pathogens.
