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Full Description
This volume focuses on detecting different cellular stresses, measuring pathological consequences within the cell, and investigating the role of cellular stresses in select diseases. In addition, this book reviews the crosstalk between different stress pathways, stress responses during ageing, and targeting stress for regenerative medicine. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and instructive, Stress Responses: Methods and Protocols seeks to aid scientists to further study multiple stress pathways and outcomes triggered by such stresses. In depth knowledge of cellular stress will eventually lead to the development of novel therapeutics to prevent or treat related diseases.
Contents
Methods for Studying ER Stress and UPR Markers in Human Cells.- Assays for Induction of the Unfolded Protein Response and Selective Activation of the Three Major Pathways.- Assays to Characterize Molecular Chaperone Functions in vitro.- Analysis of Heat Shock Factor Complex in Mammalian HSP70 Promoter.- Immunofluorescence-Based Methods to Monitor DNA End Resection.- Visualizing the Spatiotemporal Dynamics of DNA Damage in Budding Yeast.- Detecting Reactive Oxygen Species by Immunohistochemistry.- Investigating Inflammasome Activation Under Conditions of Cellular Stress and Injury.- Methods for Studying microRNA Functions During Stress.- Measuring Autophagy in Stressed Cells.- Detection of Apoptosis Using Fluorescent Probes.- Measuring Death of Pancreatic Beta Cells in Response to Stress and Cytotoxic T Cells.- Adaptation of the Secretory Pathway in Cancer Through IRE1 Signaling.- Studying Nitrosative Stress in Parkinson's Disease.- Crosstalk Between ER Stress, Oxidative Stress, and Inflammation in Health and Disease.- Stress Responses During Ageing: Molecular Pathways Regulating Protein Homeostasis.- Targeting Stress Responses for Regenerative Medicine.
