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Full Description
Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.
Contents
Probing Regional Mechanical Properties of Embryonic Tissue Using Microindentation and Optical Coherence Tomography.- Hemodynamic Flow Visualization of Early Embryonic Great Vessels using mPIV.- Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular Morphogenesis.- Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis.- Imaging the Dorsal-Ventral Axis of Live and Fixed Drosophila Melanogaster Embryos.- Light-Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit Fly.- Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis.- A Multiplex Fluorescent in situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis.- Active Cell and ECM Movements During Development.- 3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial Invasion.- Culture of Mouse Embryonic Foregut Explants.- Investigating human vascular tube morphogenesis and maturation using endothelial cell-pericyte co-cultures and a doxycycline-inducible genetic system in 3D extracellular matrices.- Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues.- Probing Cell Mechanics with Sub-Cellular Laser Dissection of Actomyosin Networks in the Early Developing Drosophila Embryo.- UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in vivo and ex vivo.- Measurement of Intercellular Cohesion by Tissue Surface Tension.- Quail-Chick Chimeras and Eye Development.- Studying Epithelial Morphogenesis in Dictyostelium.- Primary Cell Cultures of Regenerating Holothurian Tissues.- Large-Scale Parameter Studies of Cell-Based Models of Tissue Morphogenesis Using CompuCell3D or Virtual Leaf.- Simulating Tissue Morphogenesis and Signaling.- Elasticity-Based Targeted Growth Models of Morphogenesis.
