Full Description
Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.
Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.
Contents
Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre-loxP Site-Specific Recombination.- Generation of Conditional Knockout Mice.- Generating Mouse Lines for Lineage Tracing and Knockout Studies.- Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE).- Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection.- Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases.- Intra cytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice.- Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice.- Generation of Genetically Engineered Mice by the piggyBac Transposon System.- Generation and Applications of MADM-Based Mouse Genetic Mosaic System.- Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System.- In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis.- Isolation and Handling of Mouse Embryonic Fibroblasts.- Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue.- Pdx1 (GFP/w) Mice for Isolation, Characterization and Differentiation of Pancreatic Progenitor Cells.- Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development.- Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System.- Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo.- Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts.- Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment.- Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty.- Generating Double Knockout Mice to Model Genetic Intervention for DiabeticCardiomyopathy in Humans.- Experimental Osteoarthritis Models in Mice.- Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI).- Mouse Models of Acute and Chronic Colitis.- Manipulation and Assessment of Gut Microbiome for Metabolic Studies.
